The ribeirao Sucuri, a tributary of the rio Tiete, and part of the upper rio Parana basin, is located in the municipality of Pongai, Sao Paulo state. The ichthyofauna of ribeirao Sucuri was sampled at nine collection sites in May 2018. Our study captured 408 specimens representing five orders, 11 families, and 35 species. Among the species collected, two have not been previously reported from the rio Tiete basin: Eigenmannia guairaca Peixoto, Dutra & Wosiacki, 2015 and Hoplias misionera Rosso, Mabragana, Gonzalez-Castro, Delpiani, Avigliano, Schenone & Dias de Astarloa, 2016. Additionally, four species were found that are non-native: Hoplias misionera, Poecilia reticulata Peters, 1859, Roeboides descalvadensis Fowler, 1932, and Satanoperca sp. This study represents the first fish inventory of a tributary on the left margin of the Tiete-Batalha sub-basin and only the second for this portion of the rio Tiete. Our results increase to 55 the number of species recorded from this sub-basin and add data on the putative morphological variation in several species. Keywords Diversity, inventory, Neotropical freshwater fishes, Tiete-Batalha sub-basin Academic editor: Sarah Steele | Received 24 December 2019 | Accepted 12 May 2020 | Published 16 June 2020 Citation: Reia L, Costa e Silva GS, Ayala JG, Vicensotto AMPF, Benine RC (2020) Ichthyofauna of the ribeirao Sucuri, a tributary of the rio Tiete, upper rio Parana basin, southeastern Brazil. Check List 16 (3): 711-728. https://doi.org/10.15560/16.3.711