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Early development of grateloupia turuturu (Halymeniaceae, Rhodophyta)

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Abstract

Grateloupia turuturu is a commercial red alga with potential value in nutraceuticals and pharmaceuticals. To supplement information on its life history and verify whether carpospores can be used for seedling culture, early development of G. turuturu was investigated under culture conditions (27°C, 10–13 μol/(m2·s) in irradiance, photoperiod 10:14 h L:D). Three physiological stages were recognized by continuous microscopic observation: division stage, discoid crust stage, and juvenile seedling stage. At the beginning of the division stage, the carpospores developed germ tubes into which the carpospore protoplasm was evacuated, and then the carpospore protoplasm in the germ tubes began to divide continuously until discoid crusts formed. Finally, upright thalli appeared on the discoid crusts and developed into juvenile seedlings. It took about 60 days for carpospores to develop into juvenile seedlings. The growth parameters, including germination rate for carpospores and discoid crust diameter, were recorded. These results contribute more information on the life cycle, and at the same time are of great significance in the scaling-up of artificial seedling cultures of G. turuturu.

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References

  • Aguirre-Lipperheide M, Estrada-Rodriguez F J, Evans L V. 1995. Facts, problems and needs in seaweed tissue culture: an appraisal. J. Phycol., 31: 677–688.

    Article  Google Scholar 

  • Barreiro R, Quintela M, Bárbara I, Cremades J. 2006. RAPD differentiation of Grateloupia lanceola and the invasive Grateloupia turuturu (Gigartinales, Rhodophyta) in the Iberian Peninsula. Phycologia, 45(2): 213–217.

    Article  Google Scholar 

  • Bouzon Z L, Ouriques L C, Oliveira E C. 2006. Spore adhesion and cell wall formation in Gelidium floridanum (Rhodophyta, Gelidiales). J. Appl. Phycol., 18: 287–294.

    Article  Google Scholar 

  • Bulboa C, Macchiavello J, Véliz K, Oliveira E C. 2010. Germination rate and sporeling development of Chondracanthus chamissoi (Rhodophyta, Gigartinales) varies along a latitudinal gradient on the coast of Chile. Aquat. Bot., 92: 137–141.

    Article  Google Scholar 

  • D’Archino R, Nelson W A, Zuccarello G C. 2007. Invasive marine red alga introduced to New Zealand waters: first record of Grateloupia turuturu (Halymeniaceae, Rhodophyta). New Zeal. J. Mar. Fresh., 41: 35–42.

    Article  Google Scholar 

  • Denis C, Massé A, Fleurencea J, Jaouenb P. 2009. Concentration and pre-purification with ultrafiltration of a R-phycoerythrin solution extracted from macro-algae Grateloupia turuturu: Process definition and up-scaling. Sep. Puri. Technol., 69: 37–42.

    Article  Google Scholar 

  • Fujiwara-Arasaki T, Mino N, Kuroda M. 1984. The protein value in human nutrition of edible marine algae in Japan. Hydrobiologia, 116/117: 513–516.

    Article  Google Scholar 

  • García-Jiménez P, Robaina R R, Luque A, Tsekos I. 1996. Glycerolactivated cellular division and biosynthetic activity during growth and morphogenesis of carpospore seedlings of Grateloupia doryphora (Cryptonemiales, Rhodophyta). Phycologia, 35: 261–269.

    Article  Google Scholar 

  • García-Jiménez P, Rodrigo M, Robaina R R. 1998. Influence of plant growth regulators, polyamines and glycerol interaction on growth and morphogenesis of carposporelings of Grateloupia cultured in vitro. J. Appl. Phycol., 10: 95–100.

    Article  Google Scholar 

  • Gavio B, Fredericq S. 2002. Grateloupia turuturu (Halymeniaceae, Rhodophyta) is the correct name of the non-native species in the Atlantic known as Grateloupia doryphora. Eur. J. Phycol., 37: 349–359.

    Article  Google Scholar 

  • Glenn E P, Moore D, Fitzsimmons K, Azevedo C. 1996. Spore culture of the edible red seaweed, Gracilaria parvispora (Rhodophyta). Aquaculture, 142: 59–74.

    Article  Google Scholar 

  • Gusev M V, Tambiev A H, Kirikora N N, Shelyastina N N, Aslanyan R R. 1987. Callus formation in seven species of agarophyte marine algae. Mar. Biol., 95: 593–597.

    Article  Google Scholar 

  • Harlin M M, Villalard-Bohnsack M. 2001. Seasonal dynamics and recruitment strategies of the invasive seaweed Grateloupia doryphora (Halymeniaceae, Rhodophyta) in Narragansett Bay and Rhode Island Sound, Rhode Island, USA. Phycologia, 40: 468–474.

    Article  Google Scholar 

  • Huang W, Fujita Y. 1997. Callus induction and thallus regeneration in some species of red algae. Phycol. Res., 45: 105–111.

    Article  Google Scholar 

  • Huang W, Fujita Y, Ninomiya M, Ohno M. 1999. Seed production and cultivation of Grateloupia turuturu (Cryptonemiales, Rhodophyta) by callus culture. Bull. Mar. Sci. Fish., Kochi Univ., 19: 1–7.

    Google Scholar 

  • Iima M, Kinoshita T, Kawaguchi S, Migita S. 1995. Cultivation of Grateloupia acuminata (Halymeniaceae, Rhodophyta) by regeneration from cut fragments of basal crusts and upright thalli. J. Appl. Phycol., 7: 583–588.

    Article  Google Scholar 

  • Ishikawa Y. 1984. New seaweed resources 2: cultivation of Grateloupia filicina. Suisan no Kenkyu, 3: 57–60. (in Japanese)

    Google Scholar 

  • Kawaguchi S. 1997. Taxonomic notes on the Halymeniaceae (Gigartinales, Rhodophyta) from Japan. III. Synonymization of Pachymeniopsis Yamada in Kawabata with Grateloupia C. Agardh. Phycological Research, 45: 9–21.

    Article  Google Scholar 

  • Kraft G T. 1977. The morphology of Grateloupia intestinalis from New Zealand, with some thoughts on generic criteria within the family Cryptonemiaceae (Rhodophyta). Phycologia, 16: 43–51.

    Article  Google Scholar 

  • Lafontaine N, Mussio I, Rusig A M. 2011. Production and regeneration of protoplasts from Grateloupia turuturu Yamada (Rhodophyta). J. Appl. Phycol., 23(1): 17–24.

    Article  Google Scholar 

  • Mantri V A, Thakur M C, Kumar M, Reddy C R K, Jha B. 2009. The carpospore culture of industrially important red alga Gracilaria dura (Gracilariales, Rhodophyta). Aquaculture, 297: 85–90.

    Article  Google Scholar 

  • Marston M, Villalard-Bohnsack M. 2002. Genetic variability and potential sources of Grateloupia doryphora (Halymeniaceae, Rhodophyta), an invasives species in Rhode Island waters (USA). J. Phycol., 38: 649–658.

    Article  Google Scholar 

  • Mathieson A C, Dawes C J, Pederson J, Gladych R A. 2008a. The Asian red seaweed Grateloupia turuturu (Rhodophyta) invades the Gulf of Maine. Biol. Invasions, 10: 985–988.

    Article  Google Scholar 

  • Mathieson A C, Pederson J R, Neefus C D, Dawes C J, Bray T L. 2008b. Multiple assessments of introduced seaweeds in the Northwest Atlantic. J. Mar. Sci., 65: 730–741.

    Google Scholar 

  • Migita S. 1988. Cultivation of Grateloupia filicina (Rhodophyta, Cryptonemiales) by regeneration of crusts. Nippon Suisan Gakkaishi, 54: 1 923–1 927. (in Japanese)

    Article  Google Scholar 

  • Miyazawa K, Ito K. 1974a. Isolation of L-Methionine-lsulfoxide and N-Methylmethionine Sulfoide from a red alga Grateloupia turuturu. Bull. Jpn. Soc. Sci. Fish., 40: 655–660.

    Article  Google Scholar 

  • Miyazawa K, Ito K. 1974b. Isolation of a New Peptide, Lcitrulliny- L-arginine, from a red alga Grateloupia turuturu. Bull. Jpn. Soc. Sci. Fish., 40: 815–818. (in Japanese)

    Article  Google Scholar 

  • Pang S J, Xiao T, Shan T F, Wang Z F, Gao S Q. 2006. Evidences of the intertidal red alga Grateloupia turuturu in turning Vibrio parahaemolyticus into non-culturable state in the presence of light. Aquaculture, 260: 369–374.

    Article  Google Scholar 

  • Plouguerné E, Hellio C, Deslandes E, Véron B, Stiger-Pouvreau V. 2008. Anti-microfouling activities in extracts of two invasive algae: Grateloupia turuturu and Sargassum muticum. Bot. Mari., 51: 202–208.

    Article  Google Scholar 

  • Polne-Fuller M, Gibor A. 1986. Algal cell, callus and tissue cultures and selection of algal strains. Beih. Nova Hedwig., 83: 30–36.

    Google Scholar 

  • Ribera M A, Boudouresque C F. 1995. Introduced marine plants, with special reference to macroalgae: mechanisms and impact. Prog. Phycol. Res., 11: 187–268.

    Google Scholar 

  • Robaina R R, Garcia P, Garcia-Reina G, Luque A. 1990a. Morphogenetic effect of glycerol on tissue cultures of the red seaweed Grateloupia doryphora. J. Appl. Phycol., 2: 137–143.

    Article  Google Scholar 

  • Robaina R R, Garcia-Reina G, Luque A. 1990b. The effects of the physical characteristics of the culture medium on the development of red seaweeds in tissue culture. Hydrobiologia, 204/205: 137–142.

    Article  Google Scholar 

  • Robaina R R, Garcia-Reina G, Luque A. 1996. Light and glycerol driven development of Grateloupia doryphora (Rhodophyta) in vitro. Sci. Mar., 60: 283–289.

    Google Scholar 

  • Rodrigo M, Robaina R R. 1997. Stress tolerance of photosynthesis in sporelings of the red alga Grateloupia doryphora compared to that of Stage III thalli. Mar. Bio., 128: 689–694.

    Article  Google Scholar 

  • Sacramento A T, García-Jiménez P, Robaina R R. 2007. The polyamine spermine induces cystocarp development in the seaweed Grateloupia (Rhodophyta). Plant Growth Regul., 53: 147–154.

    Article  Google Scholar 

  • Saunders G W, Withall R D. 2006. Collections of the invasive species Grateloupia turuturu (Halymeniales, Rhodophyta) from Tasmania, Australia. Phycologia, 45(6): 711–714.

    Article  Google Scholar 

  • Simon C, Ar Gall E, Deslandes E. 2001. Expansion of the red alga Grateloupia doryphora along the coasts of Brittany (France). Hydrobiologia, 443: 23–29.

    Article  Google Scholar 

  • Simon-Colin C, Kervarec N, Pichon R, Deslandes E. 2004. Purification and characterization of 4-methanesulfinyl-2-methylamino butyric acid from the red alga Grateloupia doryphora Howe. Phytochemistry Rev., 3: 367–370.

    Article  Google Scholar 

  • Shao K S, Wang J X, Zhou B C. 2004. Production and application of filaments of Grateloupia turuturu (Halymeniaceae, Rhodophyta). J. Appl. Phycol., 16: 431–437.

    Article  Google Scholar 

  • Tseng C K. 1983. Common Seaweeds in China. Science Press, Beijing.

    Google Scholar 

  • Verlaque M, Brannock P M, Komatsu T, Villalard-Bohnsack M, Marston M. 2005. The genus Grateloupia C. Agardh (Halymeniaceae, Rhodophyta) in the Thau Lagoon (France, Mediterranean): a case study of marine plurispecific introductions. Phycologia, 44(5): 477–496.

    Article  Google Scholar 

  • Villalard-Bohnsack M, Harlin M M. 1997. The appearance of Grateloupia doryphora (Halymeniaceae, Rhodophyta) on the northeast coast of North America. Phycologia, 36: 324–328.

    Article  Google Scholar 

  • Villalard-Bohnsack M, Harlin M M. 2001. Grateloupia doryphora (Halymeniaceae, Rhodophyta) in Rhode Island waters (USA): geographical expansion, morphological variations and associated algae. Phycologia, 40: 372–380.

    Article  Google Scholar 

  • Wang A H, Wang J C, Duan D L. 2006. Early development of Chondrus ocellatus Holm (Gigartinaceae, Rhodophyta). Chin. J. Oceanol. Limnol., 24(2): 129–133.

    Article  Google Scholar 

  • Yokoya N S, Guimarães S M P B, Handro W. 1993. Development of callus-like structures and plant regeneration in thallus segments of Grateloupia filiformis Kützing (Rhodophyta). Hydrobiologia, 260/261: 407–413.

    Article  Google Scholar 

  • Yokoya N S, Handro W. 1996. Effects of auxins and cytokinins on tissue culture of Grateloupia dichotoma (Gigartinales, Rhodophyta). Hydrobiologia, 326(327): 393–400.

    Article  Google Scholar 

  • Zhao F J, Zhao Z G, Wang A H, Liu J D, Duan D L. 2010. Carpospore early development and callus-like tissue induction of Chrysymenia wrightii (Rhodymeniaceae, Rhodophyta) under laboratory conditions. J. Appl. Phycol., 22: 195–202.

    Article  Google Scholar 

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Authors and Affiliations

  1. College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China

    Gaoge Wang  (王高歌), Chunmei Jiang  (姜春梅), Shasha Wang  (王莎莎) & Xiaojiao Wei  (魏晓娇)

  2. Shandong Key Laboratory of Eco-environmental Science for Yellow River Delta, Binzhou University, Binzhou, 256600, China

    Fengjuan Zhao  (赵凤娟)

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  1. Gaoge Wang  (王高歌)
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  2. Chunmei Jiang  (姜春梅)
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  3. Shasha Wang  (王莎莎)
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  4. Xiaojiao Wei  (魏晓娇)
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  5. Fengjuan Zhao  (赵凤娟)
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Correspondence to Gaoge Wang  (王高歌) or Fengjuan Zhao  (赵凤娟).

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Supported by the Natural Science Foundation of Shandong Province, China (No. Y2007D42)

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Wang, G., Jiang, C., Wang, S. et al. Early development of grateloupia turuturu (Halymeniaceae, Rhodophyta). Chin. J. Ocean. Limnol. 30, 264–268 (2012). https://doi.org/10.1007/s00343-012-1071-5

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