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Studies on Proximate Composition and Phytochemical Profiling of Turbinaria ornata and its Antiproliferative Effect on Y79 Cell Lines

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Abstract

Marine brown algae Turbinaria ornata (T. ornata) that grows along Tamilnadu coast (Gulf of Mannar) were collected to study the composition of major and minor nutrients, phytochemical constituents and to evaluate the cytotoxic effect. Proximate composition of this brown seaweed was investigated by the determination of moisture, ash, protein, lipid and carbohydrate. The mineral content was analyzed using Inductive Coupled Plasma- Mass Spectrometry (ICP-MS). In general, the results of proximate analysis showed that moisture content (83.62%) was most abundant followed by carbohydrate (43.7% dry weight), ash content (23.4%) protein (5.4% dry weight) and lipid (1.9% dry weight). This work presents the data on the mineral content such as manganese, sodium, magnesium, potassium, zinc etc. of the brown algae. The chemical components present in the methanolic extract of the sample were analyzed using Gas Chromatography–Mass Spectrometer (GC-MS). The presence of secondary metabolites illustrate that this brown seaweed T. ornata can be harnessed for their biomedical potentials. Apart from this, the methanolic extract extracted also showed cytotoxic activity against human retinoblastoma Y79 cell lines and the Inhibitory Concentration (IC50) was found to be at 6.37 μg/mL. Further research should be explored for the beneficial application of T. ornata as a potent therapeutic tool against cancer.

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References

  • Abramson DH (2005) Retinoblastoma in the 20th century: past success and future challenges the Weisenfeld lecture. Invest Ophthalmol Vis Sci 46:2683–2691

    Article  Google Scholar 

  • Acremi N, .Cappoen D, Anthonissen R, Verschaeve L, Bouraoui A (2017) Phytochemical and in vitro antimicrobial and genotoxic activity in the brown algae Dictyopteris membranacea. S Afr J Bot 108:308–314

    Article  Google Scholar 

  • Ahmad F, Sulaiman MR, Saimon W, Yee CF, Matanjun P (2012) Proximate compositions and total phenolic contents of selected edible seaweed from Semporna, Sabah, Malaysia. Borneo Science 31:85–95

    Google Scholar 

  • AOAC (1997) Official methods of analysis, 16th edn. Association of Official Analytical Chemists, AOAC International, Arlington,VA, USA

  • Azmat R, Hayat A, Khanum T, Talat R, Uddin F (2006) The inhabitation of bean plant metabolism by cd metal and atrazine III: effects of seaweed Codium iyengaria on metal, herbicide toxicity and rhizosphere of the soil. Biotechnol 5:85–89

    Article  Google Scholar 

  • Black WAP (1948) The seasonal variation in chemical composition of some of the littoral seaweeds common to Scotland. Part I. Ascophyllum nodosum. J Soc Chem Ind 67:355–367

    Article  Google Scholar 

  • Black WAP (1949) Seasonal variation in chemical composition of some of the littoral seaweeds common to Scotland. Part II. Fucus serratus, Fucus Vesiculosus. Fucus spiralis and pelvetia canaliculata. J Soc Chem Ind 68:183–189

    Article  Google Scholar 

  • Blunden G (1993) Marine algae as sources of biologically active compounds. Interdiscip Sci Rev 18:73–80

    Article  Google Scholar 

  • Boo HG, Hyun JH, Kim SC, Kang JI, Kim MK, Kim SY, Cho H, Yoo ES, Kang HK (2011) Fucoidan from Undaria pinnatifida induces apoptosis in A549 human lung carcinoma cells. Phytother Res 25:1082–1086

    Article  Google Scholar 

  • Burtin P (2003) Nutritional value of seaweeds. Elec J Env Agricult Food Chem 2:498–503

    Google Scholar 

  • Chakraborty K, Paulraj R (2010) Sesquiterpenoids with free radical scavenging properties from marine macroalga Ulvafasciata delile. Food Chem 122:31–41

    Article  Google Scholar 

  • Chakraborty S, Santra SC (2008) Biochemical composition of eight benthic algae collected from Sunderban. Indian J Mar Sci 37:29–332

    Google Scholar 

  • Chapman VJ, Chapman DJ (1980) Seaweeds and their uses, 3rd edn, Chapman and Hall, Ltd., London, pp 279–312

  • Chidambaram K and Unny MM (1953) Note on the value of seaweeds as manure. In 1st Int. seaweed Symp 67–68

  • Critchley AT, Ohno M (eds) (1998) Seaweed resources of the world, Japan International Cooperation Agency, Yokosuka, Japan, pp 429

  • Cumashi A, Ushakova NA, Preobrazhenskaya ME et al (2007) A comparative study of the anti-inflammatory, anticoagulant, antiangiogenic, and antiadhesive activities of nine different fucoidans from brown seaweeds. Glycobiology 17:541–552

    Article  Google Scholar 

  • Darcy-Vrillon B (1993) Nutritional aspects of the developing use of marine macroalgae for the human food industry. Int J Food Sci Nutr 44:23–35

    Google Scholar 

  • De Aragao AP, De Oliveira TM, Quelemes PV, Perfeito MLG, Araujo MC, Santiago JDAS, Da Silva DA (2016) Green synthesis of silver nanoparticles using the seaweed Gracilaria birdiae and their antibacterial activity. Arab J Chem. https://doi.org/10.1016/j.arabjc.2016.04.014

  • Deepak P, Sowmiya R, Balasubramani G, Perumal P (2017) Phytochemical profiling of Turbinaria ornata and its antioxidant and anti-proliferative effects. J Taibah Univ Med Sci 12:329–337. https://doi.org/10.1016/j.jtumed.2017.02.002

    Google Scholar 

  • De Quirós ARB, Frecha-Ferreiro S, Vidalpérez AM, López-Hernández J (2010) Antioxidant compounds in edible brown seaweeds. Eur Food Res Technol 231:495–498

  • El Shafay SM, Ali SS, El-Sheekh MM (2016) Antimicrobial activity of some seaweeds species from Red Sea, against multidrug resistant bacteria. Egypt J Aquat Res 42:65–74

    Article  Google Scholar 

  • Frías S, Conde JE, Rodríguez MA, Dohnal V, Pérez-Trujillo JP (2002) Metallic content of wines from the Canary Islands (Spain). Application of artificial neural networks to the data analysis. Nahrung 46:370–375

    Article  Google Scholar 

  • Ghosh T, Chattopadhyay K, Marschall M, Karmakar P, Mandal P, Ray B (2009) Focus on antivirally active sulfated polysaccharides: from structure-activity analysis to clinical evaluation. Glycobiology 19:2–15

    Article  Google Scholar 

  • Gunasekaran S, Shanmugasokan VKTLS, Pitchai S, Yesudas R, Chacko A (2017) Screening of in vitro cytotoxic activity of brown seaweeds against hepatocellular carcinoma. Journal of Applied Pharmaceutical Science 7:051–055

    Google Scholar 

  • Harborne JB (1973) Phytochemical methods. Chapman and Hall Ltd, London, pp 49–188

    Google Scholar 

  • Hedge JE and Hofreiter BT (1962) In: Carbohydrate Chemistry, 17 (Eds. Whistler RL and Be Miller JN), Academic Press, New York, 420

  • Kelman D, Posner EK, McDermid KJ, Tabandera NK, Wright PR, Wright AD (2012) Antioxidant activity of Hawaiian marine algae. Mar Drugs 10:403–416

    Article  Google Scholar 

  • Kiratli H, Kocabeyoğlu S, Bilgiç S (2007) Severe pseudo–preseptal cellulitis following sub–Tenon’s carboplatin injection for intraocular retinoblastoma. Journal of AAPOS 11:404–405

    Article  Google Scholar 

  • Kumar CS, Ganesan P, Suresh PV, Bhaskar N (2008) Seaweeds as a source of nutritionally beneficial compounds-a review. J Food Sci Technol 45:1–13

    Google Scholar 

  • Liu L, Heinrich M, Myers S, Dworjanyn SA (2012) Towards a better understanding of medicinal uses of the brown seaweed Sargassum in traditional Chinese medicine: a phytochemical and pharmacological review. J Ethnopharmacol 142:591–519

    Article  Google Scholar 

  • Lowry OH, Rosenbrough NH, Farr AL, Randall RJ (1951) Protein measurements with Folin phenol reagent. J Biol Chem 193:265–275

    Google Scholar 

  • Maftuch KI, Adam A, Zamzami I (2016) Antibacterial effect of Gracilaria verrucosa bioactive on fish pathogenic bacteria. Egypt J Aquat Res 42:405–410

    Article  Google Scholar 

  • Manosroi J, Sainakham M, Manosroi W, Manosroi A (2012) Antiproliferative and apoptosis induction activities of extract from Thai medicinal plant recipes selected from MANOSROI II database. J Ethnopharmacol 141:451–459

    Article  Google Scholar 

  • Marsham S, Scott GW, Tobin ML (2007) Comparison of nutritive chemistry of a range of temperate seaweeds. Food Chem 100:1331–1336

    Article  Google Scholar 

  • Marudhupandi T, Ajith Kumar TT, Lakshmanasenthil S, Suja G, Vinothkumar T (2015) In vitro anticancer activity of fucoidan from Turbinaria conoides against A549 cell lines. Int J Biol Macromol 72:919–923

    Article  Google Scholar 

  • Matanjun P, Mohamed S, Mustapha NM, Muhammad K (2009) Nutrient content of tropical edible seaweeds, Eucheuma cottonii, Caulerpa lentillifera and Sargassum polycystum. J Appl Phycol 21:75–80

    Article  Google Scholar 

  • McDermid KJ, Stuercke B (2003) Nutritional composition of edible Hawaiian seaweeds. J Appl Phycol 15:513–524

    Article  Google Scholar 

  • Michalak I, Dmytryk A, Smieszek A, Marycz K (2017) Chemical characterization of Enteromorpha prolifera extract obtained by enzyme-assisted extraction and its influence on the metabolic activity of Caco-2. Int J Mol Sci 18. https://doi.org/10.3390/ijms18030479

  • Naru J, Aggarwal R, Mohanty AK, Singh U, Bansal D, Kakkar N, Agnihotri N (2017) Identification of differentially expressed proteins in retinoblastoma tumors using mass spectrometry-based comparative proteomic approach. J Proteome 159:77–91

    Article  Google Scholar 

  • Ortiz J, Romero N, Robert P, Araya J, Lopez-Hernández J, Bozzo C, Navarrete E, Osorio A, Rios A (2006) Dietary fiber, amino acid and tocopherol contents of the edible seaweeds Ulva lactuca and Durvillaea antarctica. Food Chem 99:98–104

    Article  Google Scholar 

  • Portugal TR, Ladines EO, Ardena SS (1983) Nutritive value of some Philippine seaweeds, part 2: proximate amino acid and vitamin composition. Philipp J Nutr 36:166–172

    Google Scholar 

  • Prince JS, Daly EL (1981) The ecology of Sargassum pteropleuron Grunow (Phaeophyceae, Fucales) in the waters off South Florida. IV. Seasonal variation in manni-Tol, protein, ash and laminaran. Phycologia 20:232–241

    Article  Google Scholar 

  • Rohani-Ghadikolaei K, Abdulalian E, Ng W-K (2012) Evaluation of the proximate, fatty acid and mineral composition of representative green, brown and red seaweeds from the Persian Gulf of Iran as potential food and feed resources. J Food Sci Technol 49:774–780

    Article  Google Scholar 

  • Rupérez P (2002) Mineral content of edible marine seaweeds. Food Chem 79:23–26

    Article  Google Scholar 

  • Śmieszek A, Giezek E, Chrapiec M, Murat M, Mucha A, Michalak I, Marycz K (2017) The Influence of Spirulina platensis Filtrates on Caco-2 Proliferative Activity and Expression of Apoptosis-Related microRNAs and mRNA. Mar Drugs 15. https://doi.org/10.3390/md15080237

  • Smit AJ (2004) Medicinal and pharmaceutical uses of seaweed natural products: a review. J Appl Phycol 16:245–262

    Article  Google Scholar 

  • Stella Mary J, Vinotha P, Pradeep AM (2012) Screening for In vitro cytotoxic activity of seaweed, Sargassum sp. against Hep-2 and MCF-7 Cancer cell. Asian Pac J Cancer Prev 13:6073–6076

    Article  Google Scholar 

  • Thirunavukkarasu R, Pandiyan P, Balaraman D, Subaramaniyan K, Edward Gnana Jothi GE, Manikkam S, Sadaiyappan B (2013) Isolation of bioactive compound from marine seaweeds against fish pathogenic bacteria Vibrio alginolyticus (VA09) and characterisation by FTIR. Journal of Coastal Life Medicine 1:26–33

    Google Scholar 

  • Trease GE, Evans WC (1989) Pharmacognosy, 13th edn. Balliere Tindall, London, pp176–180

  • Unnikrishnan PS, Suthindhiran K, Jayasri MA (2014) Inhibitory potential of Turbinaria ornata against key metabolic enzymes linked to diabetes. Biomed Res Int 2014:1–10. https://doi.org/10.1155/2014/783895

    Article  Google Scholar 

  • Vijayakrishnan R, Shields CL, Ramasubramanian A, Emrich J, Rosenwasser R, Shields JA (2010) Irradiation toxic effects during intra-arterial chemotherapy for retinoblastoma: should we be concerned? Arch Ophthalmol 128:1427–1431

    Article  Google Scholar 

  • Ye H, Wang K, Zhou C, Liu J, Zeng X (2008) Purification, antitumor and antioxidant activities in vitro of polysaccharides from the brown seaweed Sargassum pallidum. Food Chem 111:428–443

    Article  Google Scholar 

  • Zak B, Zlatkins A, Boyle (1953) A new method for the determination of serum cholesterol. J Lab Clin Med 14:486–492

    Google Scholar 

  • Zubia M, Payri CE, Deslandes E, Guezennec J (2003) Chemical composition of attached and drift specimens of Sargassum mangarevense and Turbinaria ornata (Phaeophyta: Fucales) from Tahiti, French Polynesia. Bot Mar 46:562–571

    Article  Google Scholar 

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Acknowledgements

The authors are grateful to the management and Centre for Ocean Research, Sathyabama Institute of Science and Technology for providing the support and necessary facilities in carrying out the research activity (ICP-MS & GC-MS).

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Authors and Affiliations

  1. Centre for Ocean Research, Sathyabama Institute of Science and Technology, Sathyabama University, Jeppiaar Nagar, Rajiv Gandhi Road, Chennai, Tamilnadu, 600119, India

    R. R. Remya, S. R. Radhika Rajasree, T. Y. Suman, L. Aranganathan, S. Gayathri, M. Gobalakrishnan & M. G. Karthih

  2. Department of Fish Processing Technology, v, Panangad Road, Cochin, Kerala, 682506, India

    S. R. Radhika Rajasree

  3. College of Life Science, Henan Normal University, Xinxiang, 453007, Henan, China

    T. Y. Suman

  4. Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, 400714, China

    T. Y. Suman

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  1. R. R. Remya
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Correspondence to S. R. Radhika Rajasree.

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Remya, R.R., Rajasree, S.R.R., Suman, T.Y. et al. Studies on Proximate Composition and Phytochemical Profiling of Turbinaria ornata and its Antiproliferative Effect on Y79 Cell Lines. Thalassas 35, 495–502 (2019). https://doi.org/10.1007/s41208-019-00159-x

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