Abstract
Pterocryptis anomala (Siluridae) is an endemic fish species that is distributed in the drainage basins of Southern China. In this study, we reported the complete mitochondrial genomes (mitogenomes) of two P. anomala specimens from different rivers using next-generation sequencing. The gene composition and the structural arrangement of the mitogenomes were similar to those of most other teleosts, although we examined a longer length of control region in these mitogenomes than that in most of published Siluridae mitogenomes. Phylogenetic analyses combining our novel sequences with published mitogenomes and mitochondrial cytochrome c oxidase I (COI) sequences indicated the monophyly of Pterocryptis and a sister relationship between species P. anomala and P. cochinchinensis. However, the monophyly of Pterocryptis was not very robust as a limited number of species were included in our analyses. Additionally, we uncovered that a hidden diversity might occur in P. anomala populations. The likely causes for this cryptic diversity might be the geologic movements of intense uplift of the Qinghai–Tibetan Plateau and evolution of Asian monsoons, as well as low vagility of P. anomala. Our results yielded crucially important insights into the genetic resources, phylogeny and diversity of this endemic species.
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Data availability
Two annotated mitochondrial genomes of Pterocryptis anomala (MT433098 and MT433099) and COI sequences of the four P. anomala populations (MT424989-MT425001) are available in the GenBank database.
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Acknowledgments
We are grateful for our crew help in collecting specimens. We thank Gabe Yedid, PhD, from Liwen Bianji, Edanz Editing China (www.liwenbianji.cn/ac), for editing the English text of a draft of this manuscript. This work was supported by National Key R&D Program of China (2018YFD0900902) and project of innovation team of survey and assessment of the Pearl River fishery resources (2020TD-10 and 2020ZJTD-04).
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Experiments were performed in accordance with the recommendations of the Ethics Committee of Pearl river fisheries research institute, Chinese Academy of Fishery Sciences. These policies were enacted according to the Chinese Association for the Laboratory Animal Sciences and the Institutional Animal Care and Use Committee (IACUC) protocols.
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Chen, W., Li, Y., He, Y. et al. Characterization of two complete mitochondrial genomes of Pterocryptis anomala (Siluridae) and its phylogeny and cryptic diversity. Biologia 76, 613–621 (2021). https://doi.org/10.2478/s11756-020-00582-z
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DOI: https://doi.org/10.2478/s11756-020-00582-z