Zootaxa 2715: 59–67 (2010)
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ISSN 1175-5326 (print edition)
Article
Copyright © 2010 · Magnolia Press
ZOOTAXA
ISSN 1175-5334 (online edition)
A new species of Ctenobrycon Eigenmann, 1908 (Characiformes: Characidae)
from the río Orinoco basin, Venezuela
RICARDO C. BENINE1, GUILHERME A. M. LOPES1 & ERNESTO RON2
1
Laboratório de Biologia e Genética de Peixes, Departamento de Morfologia, IBB-UNESP Campus de Botucatu, Botucatu, SP, 18600000, Brazil. E-mail: rbenine@ibb.unesp.br
2
Laboratorio de Ictiología. Escuela de Ciencias Aplicadas del Mar. Universidad de Oriente, Núcleo Nueva Esparta, Isla de Margarita,
Venezuela
Abstract
Ctenobrycon oliverai, new species, is described from the río Orinoco drainage and its main tributary, río Apure,
Venezuela. This species is readily distinguished from its congeners by the high number of series of scales above lateral
line and by morphometric differences in body depth, head length, and interorbital distance. Statistical multivariate
analysis was also conducted in order to test hypothesized differences among species of Ctenobrycon. A brief discussion
on the taxonomy of this genus is provided.
Key words: Taxonomy, Neotropical, Freshwater, Ctenobrycon spilurus
Resumo
Ctenobrycon oliverai, espécie nova, é descrita do río Orinoco e seu tributário principal, río Apure, Venezuela. A nova
espécie é prontamente distinguida de seus congêneres pelo maior número de séries de escamas acima da linha lateral e
por diferenças morfométricas observadas na altura do corpo, comprimento da cabeça e distância interorbital. Uma
análise estatística multivariada foi empregada com o objetivo de se testar as supostas diferenças entre as espécies de
Ctenobrycon. Uma breve discussão sobre a taxonomia deste gênero é apresentada.
Introduction
Ctenobrycon is a genus of small characid fishes widely distributed in the Amazon, Orinoco and Paraguay river
basins (Eigenmann, 1927; Lima et al., 2003). Currently with three valid species, C. spilurus (Vallenciennes, 1850),
C. hauxwellianus (Cope, 1870), and C. alleni (Eigenmann & McAtee, 1907) (Lima et al., 2003), Ctenobrycon was
originally proposed by Eigenmann (1908: 94) and simply described as a deeper bodied Astyanax diagnosed from
all other Tetragonopterinae by the presence of “ctenoid” scales. Subsequently, Eigenmann (1927: 330) provided a
more detailed and informative description for this genus which includes species bearing a deep and compressed
body (2.0 to 2.5 times in SL); long anal fin (39–47 total rays) with its margin nearly straight and its origin behind or
below the origin of the dorsal; mouth very small, the maxillary not reaching the eye; ctenoid scales in the preventral area, cycloid scales in the sides in young, becoming ctenoid in adults; lateral line complete (extremely
variable number of pored scales, with all possible counting from 36 to 49); caudal fin naked; maxillary with nine
(clearly an error) to two teeth; a series of tricuspid teeth in the premaxillary and an inner series of pentacuspid
teeth, whose cusps are arranged in a U-shaped curve.
Although Ctenobrycon is defined by a combination of characters which have been long discussed to have
limited phylogenetic information (see Malabarba, 1998:194 and Vari, 1998:111), it is relatively well characterized
and easily recognized among small characins (with the exception of Psellogrammus kennedyi Eigenmann, a very
similar species and putatively related to Ctenobrycon [see Mirande, 2010], from which it simply differs by
Accepted by M.R. Carvalho: 17 Nov. 2010; published: 7 Dec. 2010
59
presenting a variable poring degree of the lateral line). Nonetheless, although from the six nominal species only
three are currently considered valid, their diagnostic characters are still inconsistent, often generating major
identification problems.
Despite this, examination of collections of characid fishes in the Laboratório de Biologia e Genética de Peixes
do Instituto de Biociências da Universidade Estadual Paulista, Botucatu, has revealed an undescribed species of
Ctenobrycon from the río Orinoco basin, Venezuela. Additional samples from río Apure, a major tributary of the
río Orinoco, were located at the fish collection of the Museu de Zoologia da Universidade de São Paulo. In this
paper, we present a formal description of this new species, diagnose it from its congeners, and provided comments
on the general taxonomy of Ctenobrycon.
Material and methods
The examined material in this study is deposited in the Laboratório de Biologia e Genética de Peixes (LBP),
UNESP, Botucatu, SP and Museu de Zoologia da Universidade de São Paulo (MZUSP), São Paulo, SP.
Morphometric and meristic data for examined specimens were taken following Fink & Weitzman (1974), with the
exception of head depth (taken in the vertical through the middle of the orbit) and number of scales below lateral
line (counted between the lateral line and pelvic-fin origin). In the description, counts are followed by their
frequency in parentheses. Asterisks indicate values for the holotype. Vertebrae of the Weberian apparatus were
counted as four elements and the fused PU1+U1 as a single element from two cleared and stained (C&S)
specimens (prepared following the method of Taylor & Van Dyke, 1985).
In order to test hypothesized differences among the species of the same genus and to identify diagnostic
characters, 24 individuals of the new species of Ctenobrycon were compared with 31 individuals of C. alleni and
87 individuals of C. spilurus, using size-free canonical variate analysis (SFCVA), according to the method
developed by Reis et al. (1990). Due to its overall similarity and putative relationship, 23 individuals of
Psellogrammus kennedyi were also incorporated to this analysis. To determine which characters contribute more to
the discrimination of the species, the correlation between individual scores for the canonical variables and the
values of the characters for each individual was realized and the significance p-value from correlation used to
select the character (Strauss, 1985). The statistical procedure was carried out using the statistical package PAST
v.1.75 (Hammer et al., 2001).
Ctenobrycon oliverai, new species
Table 1, Figs. 1–2
Holotype. MZUSP 50130, 54.4 mm SL, río Apure, West of Ciudad de Apure, State of Apure, Venezuela. 28
January 1982. O. Costillo et al.
Paratypes. MZUSP 27979, 15, 38.7 – 55.8 mm SL, collected with the holotype. LBP 3061, 11, 43.8 – 54.6, 1
C&S, 50.3 mm SL, Rio Orinoco, Ciudad de Caicara del Orinoco, 07°38’11.6” N, 66°19’04.2”. State of Bolívar,
Venezuela. 03 October 2005. A. Granado & C. Oliveira.
Diagnosis. Ctenobrycon oliverai is distinguished from all congeners and Psellogrammus kennedyi by the
number of scale rows between dorsal-fin origin and lateral line (14 – 15 vs. 11 – 13 in C. spilurus; 11 – 12 in C.
alleni and P. kennedyi). Ctenobrycon oliverai is further distinguished from C. alleni by the number of humeral
blotches (one vs. two, respectively). The following characters may be usefull in distinguishing C. oliverai from
congeners: greatest body depth (48.0–58.3% of SL vs. 41.3–53.0 in C. spilurus; 41.1–51.0% of SL in C. alleni;
42.4–50.5% of SL in P. kennedyi).
Description. Morphometric data for Ctenobrycon oliverai are summarized in Table 1. Deep bodied. Greatest
body depth at dorsal-fin origin. Dorsal profile of head concave. Dorsal profile of body strongly convex from tip of
supraoccipital spine to dorsal-fin origin, dorsal-fin base posteroventrally slanted, straight or slightly convex from
posterior terminus of dorsal-fin base to end of adipose fin, and concave along caudal peduncle. Ventral body profile
convex from tip of lower jaw to anal-fin origin, anal-fin base posterodorsally slanted, concave along caudal
peduncle. Prepelvic region transversally flattened, more so proximal to pelvic-fin insertion. Postpelvic region
transversally flattened proximal to pelvic-fin insertion becoming somewhat obtuse toward anal-fin origin.
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TABLE 1. Morphometric data for Ctenobrycon oliverai, new species.
holotype
Standard length (mm)
N
paratypes
Limits
mean
55.4
28
39.4–59.9
50.1
Greatest depth
52.2
28
48.0–58.3
53.0
Snout to dorsal-fin origin
54.0
28
52.2–57.1
54.1
Snout to pectoral-fin origin
26.3
28
25.3–27.9
26.6
Snout to pelvic-fin origin
42.1
28
40.9–47.0
43.4
Snout to anal-fin origin
59.3
28
55.4–64.1
58.7
Caudal peduncle depth
10.0
24
9.2–11.7
10.1
Caudal peduncle length
2.3
23
1.6–3.0
2.2
Pectoral-fin length
23.1
28
22.2–24.6
23.4
Pelvic-fin length
17.1
28
13.4–18.5
16.9
Dorsal-fin length
35.1
28
31.9–36.5
34.2
Dorsal-fin base
14.2
28
12.5–14.7
13.3
Anal-fin length
13.0
24
10.9–16.1
13.4
Anal-fin base
49.6
27
45.9–52.0
48.8
Eye to dorsal-fin origin
43.9
28
40.9–45.8
42.9
Dorsal-fin origin to caudal–fin origin
53.3
28
52.8–57.5
54.6
Head length
24.2
28
23.2–25.7
24.7
Head depth
19.9
28
19.0–22.0
20.1
Snout length
25.2
28
21.5–26.9
24.5
Upper jaw length
35.6
28
34.6–39.8
36.3
Horizontal orbital diameter
38.6
28
37.7–43.1
40.6
Least interorbital width
41.6
28
37.4–42.8
40.2
Percentage of standard length
Percentage of head length
Mouth terminal. Maxillary not surpassing vertical through anterior margin of orbit. Premaxillary teeth in to
rows; outer row with 3 (2), 4* (25), 5 (1) tricuspid teeth, midcentral cusps longer than others; inner tooth row with
5* (27), 6 (1) teeth with 3 to 6 cusps, midcentral cusps longer than others. Maxillary with 1* (27) or 2 (1)
pentacuspidate teeth. Dentary with 5 teeth with 3 to 5 cusps usually midcentral cusps longer than others, followed
by 1 to 3 small teeth, with 1 to 3 cusps (Fig. 2).
Nostrils closer to anterior orbital margins than to each other. Supraoccipital process elongate, its tip surpasses
the vertical through origin of pectoral fin.
Dorsal-fin rays ii,9. Pectoral-fin rays i,11 (3), i,12* (17), i,13 (8). Tip of pectoral fin exceed anterior half of
length of adpressed pelvic fin. Adipose fin well developed. Pelvic-fin rays i,7, when adpressed, its tip extends up to
first branched ray of anal fin. Anal-fin rays iv, 39 (2), 40 (1), 41 (1), 42 (2), 43 (8), 44 (7), 45 (3*), 46 (1). Principal
caudal-fin rays i,17,i. Caudal fin forked.
Spinoid scales. Lateral line complete, 51 (1), 52 (2*), 53 (3), 54 (2), 55 (2), 56 (5), 58 (1). Scale rows between
dorsal-fin origin and lateral line 14 (17*), 15 (7), scale rows between lateral line and pelvic-fin origin 11 (11*), 12
(13). Circumpeduncular scale rows 18 (1*), 19(5), 20 (2), 21 (1). Scale sheath along anal-fin base in a single series,
extending posteriorly between 33–42* branched anal-fin ray.
First gill arch with 14* (12), 15 (3), 16 (1) on upper limb and 8* (11), 9 (5) on lower limb. Total vertebrae 32,
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supraneurals 4.
Sexual dimorphism. No secondary sexually dimorphic feature, such as bony hooks on anal and pelvic fins,
were observed in Ctenobrycon oliverai.
FIGURE 1. Ctenobrycon oliverai, new species, holotype, MZUSP 50130, 55.4 mm SL, río Apure, West of Ciudad de
Apure, State of Apure, Venezuela.
Color in alcohol. Overall coloration yellowish. Mid-dorsal line darker. Scattered small dark chromatophores
on dorsal surface of head from upper lip to tip of supraoccipital spine. Infraorbitals, preopercle, and opercle
retaining guanine. Lower lip well delimited by dark chromatophores. Small dark chormatophores delineating
inferior margin of eyes. Ventral portion of head with very few dark chromatophores, more concentrated on
branchiostegal rays.
Wedge-shaped humeral mark extending horizontally from fourth to sixth scale posterior to opercle and
vertically from the first to seventh scale series above lateral line. A two scales-deep silver midlateral stripe
extending on portion of body beginning at vertical through the first branched dorsal-fin ray, with stripe narrowing
posteriorly up to a vertical oval-shaped dark spot on terminus of caudal peduncle. Limits of the erector/depressor
muscles of the anal fin outlined by dark chromatophores.
Dorsal fin with scattered small dark chromatophores uniformly distributed in its interradial membrane; second
unbranched dorsal-fin ray dark pigmented with chromatophores distributed along its whole extension; first
branched dorsal-fin ray with dark pigments restricted to its distal half; further dorsal-fin rays hyaline. Anal fin with
few dark chromatophores uniformly distributed in its interradial membrane in most specimens; anal-fin hyaline or
with very few sparsely distributed dark chromatophores in few specimens. Caudal fin with small chromatophores
sparsely distributed in its interradial membrane. Adipose fin hyaline or with small chromatophores, more
concentrated in its distal half. Pectoral fins with small chromatophores homogeneously distributed along the
unbranched ray; small chromatophores in the distal half of the first six branched rays; interradial membrane
hyaline. Pelvic fin hyaline.
Distribution. Known from the río Orinoco and río Apure, río Orinoco basin, Venezuela (Fig. 3).
Etymology. The specific name is in honor of Claudio de Oliveira, the collector of the new species and a great
contributor to our knowledge of Neotropical Ichthyology.
Multivariate analysis. The results of the size-free canonical variate analysis revealed that Ctenobrycon
oliverai can be discriminated from C. alleni, C. spilurus–hauxwellianus and from P. kennedyi in the first and
second canonical variate axis that explains 68.23% and 23.22% of the total variance of the data, respectively (Fig.
4; Table 2). Ctenobrycon oliverai and C. alleni have greater anal fin-length, least interorbital width, and eye to
origin of dorsal-fin distance (higher positive values of CV1, p<0.05) than that found in C. spilurus-hauxwellianus
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BENINE ET AL.
and Psellogrammus kennedyi. These last species in turn present higher snout to dorsal-fin origin distance than C.
oliverai and C. aleni (higher negative value of CV1, p<0.05). In addition, C. oliverai could be discriminated from
the other three species on the second canonical axis, based on higher body depth (higher positive values of CV2,
p<0.05) whereas, C. spilurus-hauxwellianus, C. alleni and P. kennedyi presented greater head depth and head
length than C. oliverai (higher negative value of CV2, p<0.05).
FIGURE 2. Ctenobrycon oliverai, paratype, LBP 3061 , 50.7 mm SL, premaxillary, maxillary and dentary; left side,
lateral view (maxillary and dentary) or frontal view (premaxillary). Scale bar = 1 mm.
FIGURE 3. Map of northern South America indicating the known geographic distribution of Ctenobrycon oliverai n.
sp.. Map by M. J. Weitzman.
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TABLE 2. Loadings of variables in the first and second size-free Canonical Varietes of Ctenobrycon oliverai, C. alleni,
C. spilurus, C. hauxwellianus and Psellogrammus kennedyi.
CV1
Prob.
CV2
Prob.
ns
Body depth
0.3625
0.6293
0.3242
0.0435*
Snout to dorsal-fin origin
-0.0934
0.0115*
0.0718
0.0181*
Snout to anal-fin origin
0.1383
0.5631ns
-0.5182
0.0000*
Pelvic-fin length
0.1751
0.0749ns
0.1891
0.8191ns
Anal-fin length
0.7619
0.0000*
-0.0966
0.0620ns
Anal-fin base
0.1178
0.8807ns
0.5496
0.0978ns
Eye to dorsal-fin origin
0.1283
0.0135*
-0.2729
0.0001*
Dorsal-fin origin to caudal-fin origin
0.1385
0.3194ns
0.0673
0.1675ns
Head depth
0.2928
0.4602ns
-0.3413
0.0000*
Head length
0.1448
ns
0.9687
-0.0230
0.0026*
Least interobital width
0.2652
0.0043*
0.2115
0.3815ns
Upper jaw length
0.0523
0.2473ns
0.1822
0.1082ns
Discussion. Eigenmann (1927:330–336), in the first revisionary study after the original description of the
genus, recognized Ctenobrycon hauxwellianus (Cope), from Amazon basin; C. spilurus (Valenciennes), from
Venezuela and Suriname; and C. multiradiatus (Steindachner), from Amazon basin and possibly Paraguay basin.
This author diagnosed C. hauxwellianus from C. spilurus uniquely by the average of the body depth (2.0 times in
SL versus 2.5 times in SL, respectively). Eigenmann, however, was not sure about the validity of C. multiradiatus,
since he considered that the diagnostic characters used by Steindachner could be purely individual variations and
also pointed out that the forms from Paraguay were most likely Astyanax alleni Eigenmann & MacAtee, or A.
pelegrini Eigenmann or even Psellogrammus kennedyi Eigenmann. Géry (1977), reallocated Astyanax alleni in
Ctenobrycon but, due to the great overlap in the characters, named the subspecies C. spilurus spilurus, C. spilurus
hauxwellianus, and C. spilurus alleni. Besides, this author also cited C. multiradiatus and C. correntinus as valid,
but argued that these were probably synonyms of C. hauxwellianus and Astyanax pelegrini, respectively.
Notwithstanding the syntypes of C. spilurus and C. hauxwellianus are inadequately preserved, which further
hinders the process of delineating these species, we herein recognized C. hauxwellianus (Cope, 1870) as a junior
synonym of C. spilurus (Vallenciennes), based on the examination of the comparative lots of Ctenobrycon, which
did reveal a substantial overlap for all morphometric and meristic characters, not supporting the diagnostic
characters presented by Eigenmann (1927) for C. spilurus and C. hauxwellianus. Ctenobrycon alleni, however,
could be easily distinguished from congeners by presenting two humeral blotches versus a single one, a condition
already described by Britski, 2007.
Taking into account this great overlap in characters, a given count that falls totally out of the known range of
Ctenobrycon is strong evidence that it is related to a different species. From the 235 examined comparative
specimens, all of them presented up to 13 scale rows above the lateral line (vs. 14 –15 in C. oliverai). Moreover, no
comparative material from río Orinoco drainage has more than 12 scale rows above lateral line, which enhance our
decision of considering this species as new rather than a population which would only widen the variation range
observed for Ctenobrycon spilurus. The results of the multivariate analysis corroborate our hypothesis showing
statistically significant evidences which discriminate Ctenobrycon oliverai from C. spilurus and Psellogrammus
kennedyi.
Lima et al. (2003) listed Tetragonopterus correntinus Holmberg, T. gibbicervix, Pelegrin, T. multiradiatus
Steindachner and Astyanax pelegrini Eigenmann as species inquirendae in Ctenobrycon. Nonetheless, Mirande et
al. (2006) considered both Tetragonopterus correntinus and Astyanax pellegrini, species of Astyanax rather than
Ctenobrycon, since these do not display the spinoid scales, which promptly separate these taxa from our new
species (that presents spinoid scales). According to Mirande’s (2010) examinations, the scales of the belly of
Psellogrammus kennedyi have simple flattened serrations restricted to the margin of the scales similar to that of
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BENINE ET AL.
crenate scales in the classification of Roberts (1993). However, our examinations indicated that the form of the
scales of the belly in both Ctenobrycon and Psellogrammus species are, in fact, spinoid, with acute projections not
restricted to the margin of the scales (Fig. 5).
FIGURE 4. Scatter diagram of scores of specimens on first and second axis of Size-free Canonical Variate Analysis of
Ctenobrycon oliverai (red crosses), C. alleni (green Diamonds), C. spilurus-hauxwellianus (blue squares) and
Psellogrammus kennedyi (violet circles).
FIGURE 5. Spinoid scale from the prepelvic area of Ctenobrycon spilurus, LIRP 4999, 35.7 mm SL.
Examination of images of three syntypes of Tetragonopterus gibbicervix, made it clear that this species
presents 12 scale rows above lateral line, which distinguishes it from our new species (that presents 14–15 scale
rows above lateral line).
Géry (2006) affirmed that Tetragonopterus multiradiatus Steindachner is a junior synonym of C.
hauxwellianus, but did not expose the reasons for considering so, nor de he mention the paper where this new
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combination was proposed, if it exists at all. Considering that the syntypes of Tetragonopterus multiradiatus are
possibly lost (see Lima et al., 2003), not much information can be retrieved but from Steindachner’s original
description and putative topotypes. The eight examined specimens from the type locality (MZUSP 27765,
município de Tefé, AM, Brazil) presented one tooth in the maxillary bone, 45 to 49 scales in the lateral line, and 11
to 12 scale rows above lateral line and 9 to 10 scale rows below lateral line, perfectly fitting with C. hauxwellianus
(= C. spilurus), according to the key presented by Eigenmann. In fact, none of the herein examined morphotypes of
Ctenobrycon presented toothless maxillaries and 41–42 lateral line scales as described for T. multiradiatus by
Steindachner (1876) and, thus, there are not enough elements to assure that C. multiradiatus is a junior synonym of
C. spilurus or even if it is a species of Ctenobrycon, if we consider that no mention was made on the presence of
spinoid scales in C. multiradiatus, be it in its original description or elsewhere.
Comparative material. Ctenobrycon alleni: LIRP 3786 (n=1), Brasil, Mato Grosso do Sul, Porto Manga, rio
Paraguai; MZUSP 54023 (34) (1 C&S), Paraguai, Concepcion, Puerto Itacua, rio Paraguai; Ctenobrycon spilurus:
Brazil: MZUSP 5156 (28), Roraima, Surumu, rio Surumu; MZUSP 5601 (22), Pará, Oriximiná, rio Trombetas,
Lago Parú; Amazonas: LIRP 4999 (21) (3 C&S), Janauari, Lago Terra Preta; LIRP 4965 (10), Janauaca, Lago
Castanho; LIRP 4985 (2), Camaleão, Ilha de Marchantaria, rio Amazonas; MZUSP 27765 (8), Tefé, baixo rio
Japurá; MZUSP 54495 (8), Equador, Napo, rio Yasuní; Brazil, Acre, Cruzeiro do Sul, rio Moa: LBP 4047 (4), (1
C&S) LBP 4151 (2), rio Japiim: LBP 4095 (20); Peru, Ucayali, Província de Coronel Portilho, rio Ucayali:
MZUSP 25996 (1); MZUSP 26242 (8); Suriname, Paramaribo: ANSP 137053 (3);Venezuela, Guárico: MCP
15138 (5), Camaguán, río Portuguesa; MZUSP 74698 (3), San Fernando, río Guárico; Bolívar, Caicara Del
Orinoco: LBP 2232 (11), (2 C&S), río Orinoco; LBP 2222 (2), Laguna de Castilleros; Psellogrammus kennedyi:
LBP 3220 (24) (3 C&S), Brasil, Mato Grosso, Nobres, rio Cuiabazinho, Lagoa Marginal; Tetragonopterus alleni:
FMNH 52634, paratype, Brasil, Mato Grosso, Corumbá. Tetragonopterus gibbicervix: NMW57516, syntype,
Brazil, Amazonas, Teffé (photo), MNHN 1909-182, paratype (photo); MNHN 1909-320/321, 2 paratypes (photos);
Tetragonopterus hauxwellianus: ANSP 8138-8142, 5 paratypes, Pebas, Peru. Tetragonopterus spilurus: MNHN
5341, syntype, (photo).
Acknowledgements
We are indebted to Osvaldo T. Oyakawa (MZUSP) for curatorial assistance. José L. Figueiredo (MZUSP), Zilda
M. Lucena (MCP), Ricardo M. C. Castro, and Flávio A. Bockman (LIRP) for loan of specimens. Images of
syntypes of Tetragonopterus gibbicervix were kindly provided by Mark Sabaj Perez and Museum National
D’Histoire Naturelle through MNHN collection requests service (http://colhelper.mnhn.fr). GAML is financially
supported by CAPES. RCB is financially supported by Programa Jovem Pesquisador UNESPand FUNDIBIO. ER
is financially supported by CIUDONE.
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