AACL BIOFLUX
Aquaculture, Aquarium, Conservation & Legislation
International Journal of the Bioflux Society
Body mass-related modifications involved in
starvation at pumpkinseed Lepomis gibbosus
(Linnaeus, 1758) (Teleostei, Centrarchidae)
1
Laurențiu Burlacu, 1Cătălina F. Radu, 1Nicolae Crăciun, 1Tiberiu
Sahlean, 2Ionel-Claudiu Gavriloaie, and 3Cecilia Bucur
1
Department of Animal Biology, Faculty of Biology, University of Bucharest, Romania, EU;
2
S.C. ICPE Bistriţa S.A. (Research and Development Institute on Technologies and
Equipments for Environment Protection), Bistriţa, Romania, EU; 3Research and
Development Pisciculture Center, Nucet-Dâmboviţa, Romania, EU. Corresponding author:
L. Burlacu, laurentiuburlacu@yahoo.com
Abstract. The authors analyze the modifications observed on a population of pumpkinseed (Lepomis
gibbosus (Linnaeus, 1758)) after a 60 days period of exposure to starvation and attempt to correlate the
obtained results to other body-related characteristics, either linearly-measured (such as length, volume
and density) or weight-related, in order to achieve an estimation of a weight-loss allowance for the
species of interest, that may be interpreted, if of a low daily value, as an advantage in the invasion
process.
Key words: Lepomis gibbosus, pumpkinseed, weight-loss rate, starvation.
Resume. Les auteurs analisent les modifications observees dans une population de pumpkinseed
(Lepomis gibbosus (Linnaeus, 1758)) après une exposition au sous-alimentation pour 60 journees et
temptent de coreler les resultats avec autres caracteristiques relatifs au corps, soit elles mesuree
liniarement (comme longueur, volume et densitee corporelle) ou dependents au poids, pour estimer la
rate du perte en poids pour l’espece d’interet; cette rate, si elle a une valeur quotidienne basse, peut
etre interpretee comme un avantage pour l’espece dans le process d’invasion.
Mots-clés: Lepomis gibbosus, pumpkinseed, rate de perte en poids, sous-alimentation.
Rezumat. Autorii analizează modificările observate în urma expunerii pentru 60 zile la condiții de
înfometare a unei populații de biban-soare (Lepomis gibbosus (Linnaeus, 1758)) și încearcă să coreleze
rezultatele obținute cu alte caracteristici, fie acestea măsurate liniar (ca lungimea, volumul sau
densitatea corporală) fie dependente de greutate, pentru a obține o estimare a ratei de pierdere în
greutate pentru specia de interes, care poate fi interpretată, în cazul unei valori zilnice scăzute, drept un
avantaj în procesul de invazie.
Cuvinte cheie: Lepomis gibbosus, biban-soare, rată de pierdere în greutate, înfometare.
Introduction. Motivations for the necessity of studying the biology of foreign/invasive
species are well-known: the goal is to understand the mechanisms which facilitate the
extension of the species areal - including features which promote the invader’s capacity
to adapt to various ecosystems, higher physiological tolerance to different
factors/negative situations, higher resilience or an increased capacity to exploit a certain
niche (depending on size, trophic status, reproduction period, exposure to predators or
pathogens).
Most of these mechanisms have been thoroughly studied by Moyle & Marchetti 2006
in terms of invasive species profiling, especially in which concerns behavioral factors,
parental care, propagule pressure, prior invasion success etc. Little is known on the
impact of factors related to nutrition and growth.
Hypothetically, a lower rate of biomass loss, as a consequence of insufficient
available food or other negative factors – calculated by means of difference in initial
(higher) and final (lower) weight values, after starvation exposure, divided by number of
57
days the experiment was carried, eventually expressed under a percent form, Fulton (K)
condition factor, relative weight (Wr), volume, body density – offers the advantage of
being able to keep a survival-allowing body condition on a longer period of time, under
starvation conditions. This would constitute an important advantage in terms of invasion
potential.
Lepomis gibbosus (Linnaeus, 1758) is native to the eastern North American area
from New Brunswick (Canada) to the Florida peninsula (USA) (Scott & Grossman 1973).
In Europe, it has been introduced to many regions and river systems (Welcomme 1988).
The species has been reported in 2002 in Denmark (Jensen 2002) and in 2005 in Norway
(Sterud & Jorgensen 2006).
According to FAO (1997), it was introduced in Germany in 1881. In 1877 it was
introduced from Canada into France (Arnold 1990). In the United Kingdom it was
deliberately released into ponds as an ornamental fish (Copp et al 2002), while in Poland
an established population has been reported on the lower Oder (Heese & Przybyszewski
1985).
Introductions have been reported from the Iberian Peninsula (Sostoa et al 1987) to
Eastern and Central Europe (Welcomme 1988; de Groot 1985), including Romania
(Welcomme 1988) and the Black Sea region (Economidis et al 1981).
In most cases, the releases are thought to be deliberate, by aquarists, to populate
ponds as a decorative species; another possibility could be unintentional introductions,
along with imports of carp fry (Tandon 1976).
Material and method. The experiment was conducted on eleven specimens of Lepomis
gibbosus (L., 1758), acquired from Research and Development Pisciculture Center, Nucet
- Dâmboviţa, Romania in November, 2008. The pumpkinseed were kept for 60 days,
(from November 15, 2008 to January 13, 2009) in a 50,000cm3 (50x25x40) plastic tank,
provided with a Resun SP – 1100L internal Power Filter, indoors, at a constant
temperature of 22 – 24 Celsius degrees. During this period of time, they were not fed at
all. They were measured at the beginning and at the end of this period.
Measurements. Length and height (in centimeters) data were obtained by means of
calibrated photography: every fish was individually photographed next to a standard,
using a Panasonic DMC – L10 camera; weight (in grams) was measured using a Kern
EMB 220 – I balance with a 0.1g differential; also, every specimen’s physical volume (in
cube centimeters) was determined by displacement of a measured body of water in a
graded cylinder; two cylinders were used, both U cm3 at 20o Celsius, a larger one, graded
up to 250 cm3, with a difference between two consecutive gradations of 2 cm3, and a
smaller one, graded up to 100 cm3, with a difference between two consecutive gradations
of 1 cm3; depending on the size of each individual measured, we opted for the most
suitable of the two.
Body condition factors and indices. Considering the fact that body fat is less dense than
other body structures, we employed body density as an index of fat-loss, signaled by
increased density; correlations with obtained data were attempted, but the small number
of specimens did not allow relevant associations. Calculations focused on obtaining a
weight and weight-related loss-rate, expressed in “per diem” modifications, both as
values and in percent from initial value form.
Data processing. The photographs were processed with specially designed measurement
software, Impor Meranie, obtained from Mr. Istvan Falka. All obtained data were
introduced into table sheets and processed in Microsoft Excel 2003 with an XLSTAT patch.
Results and discussion. Initial calculations showed an average daily loss in weight of
0.016 g/day (with a minimum of 0.001g and a maximum of 0.058 g); there could not be
traced any relevant correlation between length or initial weight of the specimens and the
weight-loss rate. In percents, the pumpkinseed fish looses around 0.24% of weight per
day, in starvation conditions, with a minimum of 0.036% and a maximum of 0.59% (see
Table 1).
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Table 1
Observed initial and final weight and weight related values
Nr
1
2
3
4
5
6
7
8
9
10
11
ave
min
max
W initial
1.8
11.4
6.7
2
6.8
4.4
7
3.7
14.8
6
4.6
6.290909
1.8
14.8
W final
1.5
10.7
5.8
1.9
5.7
3.7
4.5
3.3
11.3
5.2
4.5
5.281818
1.5
11.3
delta W
0.3
0.7
0.9
0.1
1.1
0.7
2.5
0.4
3.5
0.8
0.1
1.009091
0.1
3.5
W loss/day
0.005
0.011666667
0.015
0.001666667
0.018333333
0.011666667
0.041666667
0.006666667
0.058333333
0.013333333
0.001666667
0.016818182
0.001666667
0.058333333
percentual daily weight loss
0.277777778
0.102339181
0.223880597
0.083333333
0.269607843
0.265151515
0.595238095
0.18018018
0.394144144
0.222222222
0.036231884
0.240918798
0.036231884
0.595238095
A significant correlation has been found between the daily percentual weight loss and the
difference between the initial and the final Wr (relative mass). Initial relative mass was
expressed as the ratio between the calculated mass (by means of length-weight
regression) and the observed mass of each individual. Final relative mass represented
the substitution of the initial observed mass with the final (post-starvation observed)
mass (Table 2).
Also, significant correlation was observed between differences in initial and final K
(Fulton condition factor) and daily percentual weight loss (Table 2).
Individual values for percentual daily weight loss and differences
between initial and final relative mass (Wr)
Nr
1
2
3
4
5
6
7
8
9
10
11
ave
min
max
percentual daily weight loss
0.27
0.10
0.22
0.08
0.26
0.26
0.59
0.18
0.39
0.22
0.03
0.24
0.03
0.59
Δ Wr
0.26
0.15
0.21
0.08
0.28
0.03
0.49
0.01
0.35
0.20
0.05
0.19
0.01
0.49
Table 2
ΔK
-0.01
0.05
-0.01
0.000877
0.01
-0.23
-0.10
-0.16
-0.03
-0.01
0.01
-0.04
-0.23
0.05
No significant correlation was observed between volume and volume-related calculations
(body density), pointing to the fact that all modifications depending on linear dimensions
(length, volume, density) do not involve in the condition modification derived from the
process of starvation, in opposition to other weight-related indices.
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Conclusions
Condition modification as a result of starvation processes is primarily a function of
weight and other weight related calculations;
There is no traceable relation between condition modification as a result of
starvation and linear dimensions such as length, volume or body density;
An average rate of 0.01 g per day seems to be a sufficiently slow weight-loss rate
for the species of interest, in order to allow long starvation periods and still
maintain a survival body condition; in this respect it would point to interesting
data to compare weight loss rates for other alien/invasive species, to establish a
“weight-loss allowance” rate for these species and also compare such obtained
results to similar calculations for indigenous freshwater fish species;
Weight–loss rate does not seem to be related to length or other length correlated
dimensions/measurements (such as age, for example) or initial weight of the
specimens, but it seems to have a certain relation with the body condition of the
individual (calculated either as K or Wr) prior to exposure to starvation.
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Received: 19 January 2009. Accepted: 26 January 2009. Published: 30 January 2009.
Authors:
Laurenţiu Burlacu, University of Bucharest, Faculty of Biology, Department of Animal Biology, Splaiul
Independenţei 91-95, Bucharest 050095, Romania, EU, e-mail: laurentiuburlacu@yahoo.com
Cătălina Floriana Radu, University of Bucharest, Faculty of Biology, Department of Animal Biology, Splaiul
Independenţei 91-95, Bucharest 050095, Romania, EU, e-mail: the1onlynewt@yahoo.com
Nicolae Crăciun, University of Bucharest, Faculty of Biology, Department of Animal Biology, Splaiul
Independenţei 91-95, Bucharest 050095, Romania, EU, e-mail: nicolae.craciun@yahoo.com
Tiberiu Sahlean, University of Bucharest, Faculty of Biology, Department of Animal Biology, Splaiul
Independenţei 91-95, Bucharest 050095, Romania, EU, e-mail: tiberiu.sahlean@gmail.com
Ionel-Claudiu Gavriloaie, S.C. ICPE Bistriţa S.A. - Research and Development Institute on Technologies and
Equipments for Environment Protection, Romania, Bistriţa, Parcului street, no 7, 420035, e-mail:
cgavriloaie@icpebn.ro
Cecilia Bucur, Research and Development Pisciculture Center, Romania, Dâmboviţa County, Nucet 137335,
e-mail: bucurcecilia@yahoo.com
How to cite this article:
Burlacu L., Radu C. F., Crăciun N., Sahlean T., Gavriloaie I.-C., Bucur C., 2009 Body mass-related modifications
involved in starvation at pumpkinseed Lepomis gibbosus (Linnaeus, 1758) (Teleostei, Centrarchidae).
AACL Bioflux 2(1):57-61.
Printed version: ISSN 1844-8143
Online version: ISSN 1844-9166 available at: http://www.bioflux.com.ro/docs/2009.2.57-61.pdf
© 2009 Bioflux
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Cătălina Radu