Volume 5, Nomor 2, Januari 2021 INFLUENCE OF DIFFERENT TYPES OF DIETS ON THE GROWTH PERFORMANCE OF JUVENILE GUPPIES (Poecilia reticulata Peters, 1859) 1 Abdul Khader Sultan Mohideen A K1*, Kareem Altaff K 2 Department of Zoology, The New College (Autonomous), 147, Peters Road, Affiliated to University of Madras, Chennai-600014, Tamil Nadu, India *Email: smdbio@yahoo.co.in 2 Department of Marine Biotechnology, AMET (Deemed to be University), 135, SH 49, Kanathur-603112, Tamil Nadu, India Doi: 10.31943/mangiferaedu.v5i2.105 Received: May 8, 2020 Accepted: July 25, 2020 Published: July 31, 2020 Citation: Mohideen, A. K. S., Altaff, K. (2021). Influence of Different Types of Diets on The Growth Performance of Juvenile Guppies (Poecilia reticulata peters, 1859). Jurnal Mangifera Edu, 5(2), 93-102. ABSTRACT This investigation aimed to analyze the effects of different diets and determine the optimal feed on the growth performance of guppy Poecilia reticulata (Peters, 1859). In the present study, juvenile guppies were procured from commercial fish breeders, Kolathur, Chennai, and fed with four experimental diets, namely Diet I (Control-Pellet feed), Diet II (Formulated feed), Diet III (Dry Tubifex), and Diet IV (Live feed Brachionus plicatilis) to examine the effects on growth and survival rates. In the experiment, 12 glass circular tanks with a volume of 10 L were used, and ten juveniles having an average body length and weight of 0.8 cm and 0.01 g were randomly introduced into each aquarium (80 cm×30 cm×35 cm) in triplicates. During the experimental study, the fish were fed two times a day ad libitum for 90 days. The various growth parameters and proximate composition of the diets were calculated by following standard methods. The findings showed significant differences (p<0.05) in the fish's various growth parameters fed with different diets. The fish fed with Diet IV 52% crude protein performed better than those provided with lower levels. The increase in growth parameters could be attributed to increased levels of dietary crude protein. However, the diet does not have any significant impact on fish survival rates. Thus, it is concluded that the Live Feed B. plicatilis with 52% crude protein is suitable for the optimum growth of Poecilia reticulata under the current experimental set-up and recommended for feeding juvenile guppies. Keywords: Brachionus plicatilis, Guppy fish, Live feed, Poecilia reticulata, Rotifer INTRODUCTION Ornamental fish production is an integral part of the aquaculture industry. It contributes immensely to rural development in most developing countries, and in leading markets, for aquarium fish, the retail value is several times that of its trade value, with a tremendous impact throughout the value-chain (Monticini, 2010). The global ornamental fish industry and accessories are worth $ 15 billion, and over 2 billion live ornamental fish are traded annually, while India exported ornamental fish worth $ 1.26 million, about 0.3 93 Jurnal Mangifera Edu, Volume 5, Nomor 1, Juli 2020, 93-102 percent of world trade in 2010-11 (Nair, 2012). However, India's export capacity is valued at $ 30 million, or 100 crores per annum. Besides being geared towards exports, ornamental fish dominate the market at home and abroad for a seller. India's gross domestic ornamental fish trade is reported to be close to 15 crores (Swain, 2012). Ornamental fish trade is a foreign exchange earner and provides tremendous openings to backyard and cottage industries with a significant role in the economy of developed and developing countries such as India (Mandal et al., 2007). Nevertheless, nutritional, health, and water quality issues in ornamental fish breeding need to be tackled to improve production (Ghosh et al., 2003). However, ornamental fish farming can be turned into an economically viable enterprise by developing techniques such as raceways, recirculation systems, biofiltration, and biosafety technologies. India's tropical climate, rich and diverse water resources provide the ideal conditions for the growth, maturation, and reproduction of ornamental fish. The freshwater ornamental fish species that dominate the trade are primarily from the Poecilidae, Characidae, Cyprinidae, and Cichlidae families because of their fast growth, conspicuous coloration, and market value (Kaur & Shah, 2017). The guppy Poecilia reticulata Peters 1859 is a Poeciliid fresh and brackish water ovoviviparous fish native to Trinidad, Venezuela, Barbados, Guyana, Northeast Brazil. It is one of the best-known ornamental fish used as an animal model to study ecology, evolution, developmental biology, genetics, and functional genomics. Male guppies are brilliantly colored with tremendous color variations compared to females. Vibrant male colors are a secondary sexual feature prominent and expressed as streaks, speckles, and lines of various pigmented colors with shades of black, white, red-orange, yellow, and green, whereas females do not show such coloration on their bodies (Figures 1 & 2). Female guppies show preferences for male pigmentation types such as orange, black, and distinct colored spots (Houde, 2019). However, the variation in male color patterns is due to the mutations in Kita and Csf1ra orthologs (Kottler et al., 2013). Figure 1. Male guppy Poecilia reticulata exhibiting conspicuous body pigmentation https://jurnal.biounwir.ac.id/index.php/mangiferaedu | 94 Jurnal Mangifera Edu, Volume 5, Nomor 1, Juli 2020, 93-102 Figure 2. Female guppy Poecilia reticulata showing lack of body coloration One of the most critical criteria that scientists face today in the larval rearing of fish is the change from endogenous to exogenous feeding by the fish larvae. Most of the fish larvae are unable to assimilate artificial foods during the initial stages of feeding. Hence, they depend on live food such as Rotifers, Artemia, and Copepods (Conceição et al., 2010). The rotifers belonging to the genus Brachionus have potential applications in larviculture because of their wide range in body size and better nutritive value (Hagiwara et al., 2017). The use of B. plicalitis species complex related to the mouth gape of fish larvae is administered up to 10 to 20 days of first feeding (Mills et al., 2017). After that, the other live feeds, such as Moina, are widely used to feed juvenile fishes in the ornamental fish culture (Das et al., 2012). Therefore, it is imperative to understand the nutritional demands in aquarium fish species to improve productivity (Velasco-Santamaría & Corredor-Santamaría, 2011). Tubifex is a tremendous source of food for cultured fish species. The uses of Tubifex as a feed for aquarium fishes have been reported in several studies. The fantail guppy Poecilia reticulata fed with living Tubifex attained optimal growth performance compared to commercial feed (Banani et al., 2010). The present study is an attempt to investigate the efficacy of different types of feed on the growth parameters of P. reticulata and to optimize the larval rearing conditions with emphasis on suitable feed. RESEARCH METHOD The live-bearing ornamental juvenile guppies Poecilia reticulata were procured from commercial fish breeders of Kolathur (Chennai, Tamil Nadu, India). The experiments were carried out for 90 days in triplicates in the Unit of Live Feed and Reproductive Biology Laboratory, The New College, Chennai. The juvenile guppies were shifted to rearing tanks and disinfected with 0.005 g / L of potassium permanganate solution (Qualigens) for 15 min and transferred to circular 500 L FRP tanks containing filtered tube well water. They were https://jurnal.biounwir.ac.id/index.php/mangiferaedu | 95 Jurnal Mangifera Edu, Volume 5, Nomor 1, Juli 2020, 93-102 acclimatized for ten days with aeration under laboratory conditions. The fishes were fed with commercial pellet feed (Taiyo pet products, Kolathur, Chennai) ad libitum in two divided doses. In the experiment, 12 aquarium tanks (30×40×50 cm) were used, and ten juvenile guppies for each aquarium were put in. The different water quality parameters like temperature, dissolved oxygen, and pH were observed every two days. The air diffuser increases the oxygen level by up to 9 mg L-1. Fish feed is given twice a day on an ad libitum basis. Manure and uneaten feed is removed between 9:00 am and 05.00 pm from the bottom of each container. Every day 10% of the water in each experimental tank was exchanged for a new one. Fish length and weight were documented over the ages of 10, 20, 30, 40, 50, 60, 70, 80, and 90 days from the start of the experiment. The total length (cm) of each fish was measured using a metric scale from the snout tip to the caudal fin's extended tip. After blotdrying excess water from the body, each fish's weight (g) was taken to the nearest gram using a digital monopan electronic balance. The experiments were carried out in triplicates under similar environmental conditions (Dernekbasi et al., 2010). The trials were conducted to study the effects of the dietary treatments using Diet I (Control) - Commercial pellet feed (Taiyo fish products), Diet II – Formulated feed (Table 1), Diet III – Dry Tubifex (Taiyo fish products), Diet IV – Live feed (B. plicatilis) on the growth and survival of guppies. Mean length, weight, specific growth rate (SGR), condition factor (CF), and survival were determined. The four diets tested proximate compositions were analyzed for crude protein, crude lipid, crude fiber, and ash content, using standard methods (AOAC, 2000) to verify nutrient levels. Table 1. Composition of different ingredients of the formulated feed Sl. No. Ingredients 1 2 3 4 5 6 7 Fish meal Shrimp meal Soy flour Corn flour Sago Vitamin Spirulina Contents (g/kg dry weight) 50 30 50 60 60 5 1 https://jurnal.biounwir.ac.id/index.php/mangiferaedu | 96 Jurnal Mangifera Edu, Volume 5, Nomor 1, Juli 2020, 93-102 Fish growth performance was determined by using the following formula 1, 2, and 3. Formula 1. Specific growth rate (SGR) = 𝐼𝑛 𝑊𝑡−𝐼𝑛 𝑊0 𝑡 Formula 2. Condition factor (CF) = 𝑊⁄𝐿3 × 100 Formula 3. Survival = 𝑁𝑡 𝑁0 × 100 × 100 Note: Wt and Wo are final and initial fish weights (g) Nt and No are the final and initial fish numbers for each replicate t is the experimental duration in days L is the fish length The data collected from the experiment analyzed with the help of a one-way analysis of variance (ANOVA) using SPSS Version 10.0 program to find the efficacy between the three types of feed. ANOVA revealed significant differences among groups, and hence multiple comparison tests were performed among the means using Tukey's test. The statistically significant differences were calculated by setting the aggregate type I error at 5% (p<0.05). RESULTS AND DISCUSSION The proximate composition in terms of their protein content, lipid, etc., was found to be in the following sequence Diet IV > Diet II > Diet III > Diet I (Table 2). Table 2. Proximate composition of four experimental diets Composition Crude Protein -1 38.2±0.2 -1 3.1±0.1 -1 6.2±0.2 -1 8.6±0.2 g . Kg Crude Lipid g . Kg Crude Fibre g . Kg Ash g . Kg Moisture (%) Diet II Formulated Feed Diet I Control a a 8.2±0.2 a c c b 7.2±0.2 c 10.2±0.2 46.1±0.1 5.3±0.1 8.1±0.1 b a a Diet III Dry Tubifex 42.3±0.1 3.2±0.1 8.2±0.1 6.9±0.1 Diet IV B. plicatilis b a c b 10.2±0.2 c 52.2±0.1 9.2±0.2 7.1±0.2 c b 5.2±0.1 9.2±0.1 d a b Values (Mean ± SD) with different superscripts in the same row are significantly different at the 5% level https://jurnal.biounwir.ac.id/index.php/mangiferaedu | 97 Jurnal Mangifera Edu, Volume 5, Nomor 1, Juli 2020, 93-102 The different water quality parameters like temperature, dissolved oxygen, and pH were observed to be 28±2°C, 6.31±33 mg/l, and 7-8, respectively. The statistical analysis showed significant differences between the four experimental diets. The significance level was found to be below α value 0.05. The various growth parameters and survival rates in the guppy are represented in Table 3. Juvenile guppies fed with Diet IV (B. plicatilis) registered the best growth parameters and survival rate (97%) compared to other diets. The highest SGR value was observed in Diet IV (3.71±0.00 % /d) that increased significantly with the level of dietary protein. Table 3. Growth Performance and survival rate of Poecilia reticulata fed with different diets for 90 days Diet II Diet III Diet IV Formulated Dry Tubifex B. plicatilis Feed Initial Length (cm) 0.8±0.05 0.8±0.05 0.8±0.05 0.8±0.05 a b ab Final Length (cm) 2.86±0.049 2.91±0.061 2.88±0.042 3.00±0.113c Initial Weight (g) 0.01±0.00 0.01±0.00 0.01±0.00 0.01±0.00 a c b Final Weight (g) 0.226±0.002 0.238±0.002 0.231±0.002 0.281±0.004d SGR (%) 3.47±0.005a 3.52±0.005c 3.49±0.005 b 3.71±0.005 d Condition factor (CF) 0.950±0.01a 0.962±0.02a 0.969±0.01a 1.039±0.02b Survival (%) 93.33±5.77a 93±5.77a 97±5.77a 97±5.77a Values (Mean±SD) with different superscripts in the same row are significantly different at Growth Performance Diet I Control the 5% level The final mean length (3.00±0.113 cm) and weight (0.281±0.004 g) were highest in Diet IV than other diets. The enhanced growth performance of fish fed with live feed rotifer may be due to their small size, better nutrition, and feed acceptability, which agree with Ogata et al. (2011) studies. The rotifer diet's impact on pikeperch (Sander lucoperca) significantly improved pikeperch larvae's optimum survival rate (Yanes-Roca et al., 2018). According to Gopakumar et al. (2012) the fish larvae are typically attracted to live feed due to their movement and exhibit a preference for small-size prey concerning their mouth gape. However, the effective use of live feed mainly depends on its size and nutritional content. The role of B. plicatilis species in the aquaculture sector is an essential factor in improving the quality of food supply and the growth of larviculture (Kim et al., 2018). Besides, experimental reports suggest that no single formulated artificial fish diet can provide better growth and survival rates than live feed despite advancements in fish feed technology. Thus, https://jurnal.biounwir.ac.id/index.php/mangiferaedu | 98 Jurnal Mangifera Edu, Volume 5, Nomor 1, Juli 2020, 93-102 the higher growth efficiency in P. reticulata could be due to 52% crude protein and 9% crude lipid levels in the live feed rotifer. The juvenile guppies fed with Diet II (Formulated feed) showed better growth parameters than Diet I and Diet III. The 46% crude protein content of the diet might have enhanced the bodyweight of guppies. Similarly, Mohanta & Subramanian (2002) evaluated the growth performance of the goldfish Carassius auratus using four formulated against locally available commercial feeds. The weight increment with commercial feed was significant compared to formulated feeds (40% crude protein-based on chicken liver, mussel meal or squid meal, lean shrimp meal). Furthermore, when the zebrafish larvae fed with formulated diet from the initial stage onwards showed an increase in the total length size (Kaushik et al., 2011). However, the formulated feed did not impact zebrafish's growth aspects (Gonzales & John, 2012). Juvenile guppies fed with Diet III (Dry Tubifex) showed reduced growth parameters compared to Diet IV and Diet II. The decreased growth performance with Dry Tubifex may be due to the diet's nature that exhibits minimal feed variation, which conforms to the results observed in zebrafish fed dried Tubifex (Rabbane & Rahman, 2017). Furthermore, the angelfish Pterohyllum scalare fed with dried Tubifex also showed lower growth performance than live food (Kasiri et al., 2012). In contrast, Görelşahin et al. (2018) established Tubifex's effects on the growth aspects of P reticulata. Their results indicated improved productivity and increased growth efficiency only in females when compared to male guppies. Lower growth performance was observed in Diet I (Pellet feed-control) when compared to other experimental diets. Similar results were observed in the larval rearing of koi carp C. carpio (Sarower-E-Mahfuj et al., 2012). The most striking disadvantages of the pellet feed are due to its size and sinking ability. However, most juvenile species may not consume the bigger pellets than their mouth size (Pandey, 2013). Nevertheless, zebrafish fed with commercial pellet feed was characterized by viable offsprings and better growth aspects under in vitro conditions (Rabbane & Rahman, 2017). Eventually, lower growth and survival rates of larvae fed with the artificial diet could be due to inefficient use of artificial dry food in the first few weeks of life (Montchowui et al., 2008). Condition factor (CF) was highest in Diet IV (1.039±0.02) compared to other groups. The CF of Diet IV was found to be 1.039 (>1), is an indication of better growth performance and good health condition of the juvenile guppies during the experimental period, which is following the length-weight relationship and condition factor observed in Botia Dario https://jurnal.biounwir.ac.id/index.php/mangiferaedu | 99 Jurnal Mangifera Edu, Volume 5, Nomor 1, Juli 2020, 93-102 (Haque & Biswas, 2014) and the Nile tilapia fingerlings Oreochromis niloticus (Ighwela et al., 2011) indicating good health status and isometric growth. CONCLUSION This study has demonstrated that live feed B. plicatilis (52% crude protein) has significantly influenced the various growth parameters and survival rate of juvenile guppies Poecilia reticulata. Moreover, it establishes the primacy of the live feed Rotifer over conventional formulated feed in promoting optimum growth and better health status of the guppies. 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