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Seminal characterization of the Amazonian fire-eye tetra Moenkhausia oligolepis (Günther, 1864)

Published online by Cambridge University Press:  19 August 2020

Jeane Rodrigues ,
Raquel Santos dos Santos ,
Jhennifer Gomes Cordeiro ,
Marissol Leite ,
Hingrid Suzzan Tarso Oliveira e Oliveira ,
Hadda Tercya ,
Bruna Patrícia Dutra Costa ,
Nivaldo Ferreira do Nascimento Open the ORCID record for Nivaldo Ferreira do Nascimento [Opens in a new window] ,
Caio Maximino  and
Diógenes Henrique de Siqueira-Silva Open the ORCID record for Diógenes Henrique de Siqueira-Silva [Opens in a new window]
Jeane Rodrigues
Affiliation:
Group of Studies on the Reproduction of Amazon fish (GERPA/LaNeC), Biology Faculty (FACBIO), University Federal of South and Southern of Pará (Unifesspa), Marabá, Pará, Brazil Post-Graduation Program in Amazonian Animal Reproduction – ReproAmazon – UFPA/Ufra Belem, Pará, Brazil
Raquel Santos dos Santos
Affiliation:
Group of Studies on the Reproduction of Amazon fish (GERPA/LaNeC), Biology Faculty (FACBIO), University Federal of South and Southern of Pará (Unifesspa), Marabá, Pará, Brazil
Jhennifer Gomes Cordeiro
Affiliation:
Group of Studies on the Reproduction of Amazon fish (GERPA/LaNeC), Biology Faculty (FACBIO), University Federal of South and Southern of Pará (Unifesspa), Marabá, Pará, Brazil PPG in Biodiversity and Biotechnology (BIONORTE)
Marissol Leite
Affiliation:
Group of Studies on the Reproduction of Amazon fish (GERPA/LaNeC), Biology Faculty (FACBIO), University Federal of South and Southern of Pará (Unifesspa), Marabá, Pará, Brazil
Hingrid Suzzan Tarso Oliveira e Oliveira
Affiliation:
Group of Studies on the Reproduction of Amazon fish (GERPA/LaNeC), Biology Faculty (FACBIO), University Federal of South and Southern of Pará (Unifesspa), Marabá, Pará, Brazil
Hadda Tercya
Affiliation:
Group of Studies on the Reproduction of Amazon fish (GERPA/LaNeC), Biology Faculty (FACBIO), University Federal of South and Southern of Pará (Unifesspa), Marabá, Pará, Brazil
Bruna Patrícia Dutra Costa
Affiliation:
Group of Studies on the Reproduction of Amazon fish (GERPA/LaNeC), Biology Faculty (FACBIO), University Federal of South and Southern of Pará (Unifesspa), Marabá, Pará, Brazil PPG in Biodiversity and Biotechnology (BIONORTE)
Nivaldo Ferreira do Nascimento
Affiliation:
Laboratory of Fish Biotechnology, National Center for Research and Conservation of Continental Fish, Chico Mendes Institute of Biodiversity Conservation, Rodovia Pref. Euberto Nemésio Pereira de Godoy, Pirassununga, SP13630-970, Brazil
Caio Maximino
Affiliation:
Group of Studies on the Reproduction of Amazon fish (GERPA/LaNeC), Biology Faculty (FACBIO), University Federal of South and Southern of Pará (Unifesspa), Marabá, Pará, Brazil PPG in Biodiversity and Biotechnology (BIONORTE)
Diógenes Henrique de Siqueira-Silva*
Affiliation:
Group of Studies on the Reproduction of Amazon fish (GERPA/LaNeC), Biology Faculty (FACBIO), University Federal of South and Southern of Pará (Unifesspa), Marabá, Pará, Brazil PPG in Biodiversity and Biotechnology (BIONORTE) Post-Graduation Program in Amazonian Animal Reproduction – ReproAmazon – UFPA/Ufra Belem, Pará, Brazil
*
Author for correspondence: Diógenes Henrique de Siqueira-Silva. Group of Studies on the Reproduction of Amazon fish (GERPA/LaNeC), Biology Faculty (FACBIO), University Federal of South and Southern of Pará (Unifesspa), Marabá, Pará, Brazil. E-mail: siqueira.diogenes@gmail.com, diogenessilva@unifesspa.edu.br
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Summary

The seminal characteristics of Moenkhausia oligolepis are described. Three males were induced with a single dose of carp pituitary. Semen was collected 6 h after induction, and diluted in dibasic sodium phosphate extender solution. For motility analysis, 1 µl of diluted semen was added to 10 µl of distilled water to achieve gamete activation. The average duration of total motility was 76.67 s; while the average sperm motility rate at intervals of 15 s was 95.3, 85.3, 59.6, 31.7, 13.0, 4.6 and 1.2%. To determine sperm concentration in samples, 0.5 μl of semen was diluted with 500 μl of glutaraldehyde. An aliquot of 10 μl of this dilution was utilized for cell counting. An average count of 4.97 × 109 ± 3.46 sperm/ml was obtained. Morphological analyses were performed using eosin–nigrosine dye; 20.33% of the sperm were observed to be dead. Live sperm, comprising the other 79.67%, had an average length of approximately 30 µm, with a head diameter of 4.488 ± 0.7 µm; and a flagella plus mid-piece length of 26.071 ± 12.4 µm. Of those sperm, 69% had a normal morphology, while 31% had primary and secondary abnormalities. The observed abnormality rate did not have a detrimental effect on artificial fertilization potential for the species. The description of the seminal characteristics of a species is one of the most important sets of information required for artificial reproduction of fish in captivity. It also contributes significantly to the total biological knowledge of the studied species.

Keywords

Amazonian fishArtificial fertilizationFish biotechnologySemen analysisSpermatozoa
Type
Research Article
Information
Zygote , Volume 28 , Issue 6 , December 2020 , pp. 453 - 458
Copyright
© The Author(s), 2020. Published by Cambridge University Press

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References

Alavi, SMH, Cosson, J, Bondarenko, O and Linhart, O (2019). Sperm motility in fishes: (III) Diversity of regulatory signals from membrane to the axoneme. Theriogenology 15, 143–65.CrossRefGoogle Scholar
Andrade, DR and Yasui, GS (2003). O manejo da reprodução natural e artificial e sua importância na produção de peixes no Brasil. Rev Bras Reprod Anim 27, 166–72.Google Scholar
Andrade, ES, Carvalho, AFS, Ferreira, MR, Paula, FG, Rodrigues, FS, Felizardo, VO, Reis Neto, RV and Murgas, LDS (2014). Indutores hormonais na reprodução artificial de curimba (Prochilodus lineatus). Rev Bras Reprod Anim 38, 230–6.Google Scholar
Baulny, BO, Le Vern, Y, Kerboueuf, D and Maisse, G (1997). Flow cytometric evaluation of mitochondrial activity and membrane integrity in fresh and cryopreserved rainbow trout (Oncorhynchus mykiss) spermatozoa. Cryobiology 34, 141–9.CrossRefGoogle Scholar
Benine, RC, Mariguela, TC and Oliveira, C (2009). New species of Moenkhausia Eigenmann, 1903 (Characiformes: Characidae) with comments on the Moenkhausia oligolepis species complex. Neotrop Ichthyol 7, 161–8.CrossRefGoogle Scholar
Billard, R (1983). Effects of coelomic and seminal fluids and various saline diluents on the fertilizing ability of spermatozoa in the rainbow trout, Salmo gairdneri. J Reprod Fertil 68, 77–84.CrossRefGoogle ScholarPubMed
Bombardelli, RA, Mörschbächer, EF, Campagnolo, R, Sanches, EA and Syperreck, MA (2006). Dose inseminante para fertilização artificial de ovócitos de jundiá cinza, Rhamdia Quelen (Quoy & Gaimardm, 1824). R Bras Zootec 35, 1251–7.CrossRefGoogle Scholar
Borges, A, Siqueira, DR, Jurinitz, DF, Zanini, R, Amaral, F, Grillo, ML, Oberst, ER and Wassermann, GF (2005). Biochemical composition of seminal plasma and annual variations in semen characteristics of jundia Rhamdia quelen (Quoy and Gaimard, Pimelodidae). Fish Physiol Biochem 31, 45–53.CrossRefGoogle Scholar
Caldas, JS and Godoy, L (2019). Sperm characterization of the endangered Amazonian fish Hypancistrus zebra: Basic knowledge for reproduction and conservation strategies. Anim Reprod Sci 204, 117–24.CrossRefGoogle Scholar
Domínguez-Castanedo, O, Toledano-Olivares, A and Ávalos-Rodríguez, A (2015). Efecto del suplemento de astaxantina sobre la calidad seminal em Moenkhausia sanctaefilomenae (Teleostei: Characidae). Lat Am J Aquat Res 43, 215–21.CrossRefGoogle Scholar
Dreanno, C, Suquet, M, Desbruyères, E, Cosson, J, Dellioud, H and Billarda, R (1998). Effect of urine on semen quality in turbot (Psetta maxima). Aquaculture 169, 247–62.CrossRefGoogle Scholar
FAO (2018). The State of World Fisheries and Aquaculture 2018. Meeting the sustainable development goals. Rome. Licence: CC BY-NC-SA 3.0 IGO.Google Scholar
Felizardo, VO, Murgas, LDS, Drumond, MM and Silva, JA (2010). Dose inseminante utilizada na fertilização artificial de ovócito de piracanjuba (Brycon orbignyanus). Rev Ceres 57, 648–52.CrossRefGoogle Scholar
Ferreira, AA, Nuñer, APO, Luz, RK, Tataje, DAR, Esquivel, JR and Restrepo, JB (2001). Avaliação qualitativa e quantitativa do sêmen do jundiá, Rhamdia quelen. B Inst Pesca 27, 57–60.Google Scholar
Froese, R and Pauly, D (2018). Moenkausya oligolepis (Günther, 1864) Glass tetra. World Wide Web electronic publication. FishBase. Disponível em: http://www.fishbase.se/Summary/SpeciesSummary.php?id=12391&lang=portuguese Access em: 29 Jan 2019.Google Scholar
Herman, HA, Mitchell, JR and Doak, GA (1994). The artificial insemination and embryo transfer of diary and beef cattle. Illinois: Interstate Publishers, 382 pp.Google Scholar
Kavamoto, ET, Mainardes-Pinto, CSR, Andrade-Talmelli, EF and Campos, BES. (1997). Produção espermática do curimbatá Prochilodus scrofa Steindachner, 1881. B Inst Pesca 24, 73–8.Google Scholar
Kavamoto, ET, Barnabe, VH, Campos, BES and Andrade-Talmelli, EF (1999). Anormalidades morfológicas nos espermatozóides do curimbatá, Prochilodus lineatus (Steindachner, 1881) (Osteichthyes, Characiformes, Prochilodontidae). B Inst Pesca 25, 61–6.Google Scholar
Kowalski, RK and Cejko, BI (2019). Sperm quality in fish: Determinants and affecting factors. Theriogenology 1, 94–108.CrossRefGoogle Scholar
Leite, LV, Melo, MAP, Oliveira, FCE, Pinheiro, JPS, Campello, CC, Nunes, JF and Salmito-Vanderley, CSB (2013). Determinação da dose inseminante e embriogênese na fertilização artificial de tambaqui (Colossoma macropomum). Arq. Bras. Med. Vet. Zootec. 65, 421–9.CrossRefGoogle Scholar
Lenz, DR, Victorio, AM, Lima, MCC, Prado, TF, Paula, FG, Meirinhos, MLG and Arnhold, E (2018). Caracterização do sêmen de tambaqui (Colossoma macropomum) durante período reprodutivo. Rev Ciênc Agrovet 17, 603–7.CrossRefGoogle Scholar
Marinho, MMF and Langeani, F (2016). Reconciling more than 150 years of taxonomic confusion: the true identity of Moenkhausia lepidura, with a key to the species of the M. lepidura group (Characiformes: Characidae). Zootaxa 4107, 338–52.CrossRefGoogle Scholar
Miller, GT and Spoolman, SE (2015). Ciência Ambiental – Cengage Learning, Tradução da 14ª edição norte-americana. Acesso em: https://issuu.com/cengagebrasil/docs/ciencia_ambiental_livreto.Google Scholar
Morisawa, M and Suzuki, K (1980). Osmolality and potassium ion: their roles in initiation of sperm motility in teleosts. Science 210, 1145–7.CrossRefGoogle ScholarPubMed
Murgas, LDS, Felizardo, VO, Ferreira, MR, Andrade, ES and Veras, GC (2011). Importância da avaliação dos parâmetros reprodutivos em peixes nativos. Rev Bras Reprod Anim 35, 186–91.Google Scholar
Narahara, MY, Andrade-Talmelli, EF, Kavamoto, ET and Godinho, HM (2002). Reprodução induzida da Pirapitinga-do-Sul, Brycon opalinus (Cuvier, 1819), mantida em condições de confinamento. R Bras Zootec 31, 1070–5.CrossRefGoogle Scholar
Paulino, MS, Murgas, LDS, Felizardo, VO and Freitas, RTF (2012). Anormalidades espermáticas de Piaractus mesopotamicus após descongelamento utilizando diferentes metodologias. Arq Bras Med Vet Zootec 64, 1591–6.CrossRefGoogle Scholar
Poleo, GA, Denniston, RS, Reggio, BC, Godke, RA and Tiersch, TR (2001). Fertilization of eggs of zebrafish, Danio rerio, by intracytoplasmic sperm injection. Biol Reprod 65, 961–6.CrossRefGoogle ScholarPubMed
Reia, L. (2018). Revisão Taxonômica das espécies do grupo Moenkhausia oligolepis (Teleostei: Ostariophysi: Characiformes). Dissertação (mestrado) – Universidade Estadual Paulista ‘Júlio de Mesquita Filho’, Instituto de Biociências de Botucatu.Google Scholar
Rurangwa, E, Kime, DE, Ollevier, F and Nash, JP (2004). The measurement of sperm motility and factors affecting sperm quality in cultured fish. Aquaculture 234, 1–28.CrossRefGoogle Scholar
Sanches, EA, Bombardelli, RA, Baggio, DM and Souza, BE (2009). Dose inseminante para fertilização artificial de ovócitos de dourado. R Bras Zootec 38, 2091–8.CrossRefGoogle Scholar
Silva, JMA, Murgas, LDS, Felizardo, VO, Pereira, GJM, Navarro, RD and Mello, RA (2009). Características seminais e índices reprodutivos de curimba (Prochilodus lineatus) em diferentes períodos reprodutivos. Rev Bras Saúde Prod An 10, 668–77.Google Scholar
Silva, AC, Galvão, JAS, Teixeira, EG and Farias, WRL (2014). Caracterização e resfriamento do sêmen de curimatã, Prochilodus brevis (Steindachnner, 1874). Rev Bras Hig Sanid Anim 8, 105–9.Google Scholar
Stockley, P, Gage, MJ, Parker, GA and Moller, AP (1997). Sperm competition in fishes: the evolution of testis size and ejaculate characteristics, Am Nat 149, 933–54.CrossRefGoogle ScholarPubMed
Streit, DP Jr, Moraes, JV, Ribeiro, RP, Cardozo, RM and Moreira, HLM (2002). As tendências da utilização do extrato de hipófise na reprodução de peixes – Revisão. Arq Ciên Vet Zool 5, 231–8.Google Scholar
Streit, DP JR, Ribeiro, RP, Moraes, GV, Mendez, LV, Gallo, JM, Digmayer, M and Povh, JA (2006). Características qualitativas do sêmen de pacu (Piaractus mesopotamicus) após indução hormonal. Biosci J 22, 119–25.Google Scholar
Streit, DP Jr, Oliveira, AC, Ribeiro, RP, Sirol, RN, Moraes, GV, Galo, JM and Digmayer, M (2009). Motilidade, vigor e patologias seminal in natura e pós criopreservação de Piaractus mesopotamicus. B Inst Pesca 35, 159–67.Google Scholar
Taborsky, M (1998). Sperm competition in fish: ‘bourgeois’ males and parasitic spawning. Trends Ecol E 13, 222–7.CrossRefGoogle ScholarPubMed
Tavares-Dias, M and Mariano, WS (2015). Aquicultura no Brasil: Novas Perspectivas. Aspectos Biológicos, Fisiológicos e Sanitários de Organismos Aquáticos 429 pp.Google Scholar