Molecular Characterization and Response of Silver Carp (Hypophthalmichthys molitrix) GLUT1 under Hypoxia Stress
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Fish
2.2. Experimental Design
2.3. Total RNA Extraction and cDNA Synthesis
2.4. Glut1 Cloning and Sequencing
2.5. Bioinformatics Analysis of glut1
2.6. Fluorescence Quantitative PCR (qPCR) Analysis
2.7. Statistical Analysis
3. Results
3.1. Glut1 Sequence Analysis
3.2. Homology and Phylogenetic Tree Analysis of Silver Carp glut1
3.3. Expression Pattern of glut1 in Different Tissues of Silver Carp
3.4. Analysis of glut1 Expression in Tissues under Different Oxygen Concentrations
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Primer Name | Primer Sequence (5′–3′) | Function |
---|---|---|
Glut1-1 | F: CCCTTGGTTTCTGGGACTT | ORF amplification |
R: ACTGGACGGTTCGTGGTAG | ||
Glut1-3GSP1 | ACTATCGGAGCTGGAGTCGTCA | 3RACE |
Glut1-3GSP2 | CTGGACCGCAAACTTTATCGTG | 3RACE |
UPM | CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT | |
UPM Short | CTAATACGACTCACTATAGGGC | |
Glut1-qPCR-F | TTGCCTTGCTGGAGAAATACG | qPCR |
Glut1-qPCR-R | CCCACGATAAAGTTTGCGGTC | qPCR |
β-actin-F | GAACCCCAAAGCCAACAG | qPCR |
β-actin-R | CAGAGTCCATCACGATACCAG | qPCR |
Species | Accession Number | Sequence Identity |
---|---|---|
Megalobrama amblycephala | KM977633.1 | 97.3% |
Ctenopharyngodon idella | MG818478.1 | 96.7% |
Sinocyclocheilus anshuiensis | XP_016312675.1 | 94.7% |
Anabarilius grahami | ROL50799.1 | 94.4% |
Labeo rohita | XP_050968139.1 | 93.7% |
Onychostoma macrolepis | KAF4112506.1 | 93.3% |
Pimephales promelas | XP_039548111.1 | 92.9% |
Myxocyprinus asiaticus | XP_051567510.1 | 91.8% |
Danio rerio | XP_002662574.1 | 91.2% |
Cyprinus carpio | AAF75683.1 | 89.4% |
Salmo trutta | XP_029550223.1 | 87.2% |
Oncorhynchus mykiss | AAF75681.1 | 86.8% |
Oreochromis niloticus | NP_001266656.1 | 81.8% |
Sparus aurata | XP_030275602.1 | 81.5% |
Gadus morhua | XP_030230417.1 | 81.4% |
Mus musculus | NP_035530.2 | 80.3% |
Homo sapiens | NP_006507.2 | 79.9% |
Ovis aries | XP_027824429.1 | 79.5% |
Gallus gallus | AAB02037.1 | 78.8% |
Drosophila melanogaster | NP_001097467.1 | 46% |
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Zhang, Z.; Li, X.; Zou, G.; Liang, H. Molecular Characterization and Response of Silver Carp (Hypophthalmichthys molitrix) GLUT1 under Hypoxia Stress. Fishes 2023, 8, 425. https://doi.org/10.3390/fishes8080425
Zhang Z, Li X, Zou G, Liang H. Molecular Characterization and Response of Silver Carp (Hypophthalmichthys molitrix) GLUT1 under Hypoxia Stress. Fishes. 2023; 8(8):425. https://doi.org/10.3390/fishes8080425
Chicago/Turabian StyleZhang, Zewen, Xiaohui Li, Guiwei Zou, and Hongwei Liang. 2023. "Molecular Characterization and Response of Silver Carp (Hypophthalmichthys molitrix) GLUT1 under Hypoxia Stress" Fishes 8, no. 8: 425. https://doi.org/10.3390/fishes8080425